Method to extract intact and pure RNA from mycobacteria
| Title | Method to extract intact and pure RNA from mycobacteria |
| Publication Type | Journal Article |
| Year of Publication | 2011 |
| Authors | Akhtar, S, Sarkar, S, Mishra, A, Sarkar, D |
| Journal | Analytical Biochemistry |
| Volume | 417 |
| Issue | 2 |
| Pagination | 286-288 |
| Date Published | OCT |
| ISSN | 0003-2697 |
| Keywords | Dormant, Hypoxia, Mycobacteria, RNA isolation, Spheroplast, Trizol |
| Abstract | We describe a high-yielding, simple, and aerosol-free protocol for the isolation of RNA from mycobacteria that does not require sophisticated instruments. The method yielded 50 mu g of RNA from 10(7) cells, 50 times more than a recently reported method. Our method can extract total RNA from aerobically grown bacteria and from in vitro hypoxia-induced dormant bacilli and mycobacteria residing within infected macrophages. (C) 2011 Elsevier Inc. All rights reserved. |
| DOI | 10.1016/j.ab.2011.06.028 |
| Type of Journal (Indian or Foreign) | Foreign |
| Impact Factor (IF) | 2.85 |
Divison category:
Biochemical Sciences