%0 Journal Article %J Analytical Biochemistry %D 2011 %T Method to extract intact and pure RNA from mycobacteria %A Akhtar, Shamim %A Sarkar, Sampa %A Mishra, Abhishek %A Sarkar, Dhiman %K Dormant %K Hypoxia %K Mycobacteria %K RNA isolation %K Spheroplast %K Trizol %X

We describe a high-yielding, simple, and aerosol-free protocol for the isolation of RNA from mycobacteria that does not require sophisticated instruments. The method yielded 50 mu g of RNA from 10(7) cells, 50 times more than a recently reported method. Our method can extract total RNA from aerobically grown bacteria and from in vitro hypoxia-induced dormant bacilli and mycobacteria residing within infected macrophages. (C) 2011 Elsevier Inc. All rights reserved.

%B Analytical Biochemistry %I ACADEMIC PRESS INC ELSEVIER SCIENCE %C 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA %V 417 %P 286-288 %8 OCT %G eng %N 2 %3 Foreign %4 2.85 %R 10.1016/j.ab.2011.06.028