TY - JOUR T1 - Method to extract intact and pure RNA from mycobacteria JF - Analytical Biochemistry Y1 - 2011 A1 - Akhtar, Shamim A1 - Sarkar, Sampa A1 - Mishra, Abhishek A1 - Sarkar, Dhiman KW - Dormant KW - Hypoxia KW - Mycobacteria KW - RNA isolation KW - Spheroplast KW - Trizol AB -

We describe a high-yielding, simple, and aerosol-free protocol for the isolation of RNA from mycobacteria that does not require sophisticated instruments. The method yielded 50 mu g of RNA from 10(7) cells, 50 times more than a recently reported method. Our method can extract total RNA from aerobically grown bacteria and from in vitro hypoxia-induced dormant bacilli and mycobacteria residing within infected macrophages. (C) 2011 Elsevier Inc. All rights reserved.

PB - ACADEMIC PRESS INC ELSEVIER SCIENCE CY - 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA VL - 417 IS - 2 U3 - Foreign U4 - 2.85 ER -