Method to extract intact and pure RNA from mycobacteria

TitleMethod to extract intact and pure RNA from mycobacteria
Publication TypeJournal Article
Year of Publication2011
AuthorsAkhtar, S, Sarkar, S, Mishra, A, Sarkar, D
JournalAnalytical Biochemistry
Date PublishedOCT
KeywordsDormant, Hypoxia, Mycobacteria, RNA isolation, Spheroplast, Trizol

We describe a high-yielding, simple, and aerosol-free protocol for the isolation of RNA from mycobacteria that does not require sophisticated instruments. The method yielded 50 mu g of RNA from 10(7) cells, 50 times more than a recently reported method. Our method can extract total RNA from aerobically grown bacteria and from in vitro hypoxia-induced dormant bacilli and mycobacteria residing within infected macrophages. (C) 2011 Elsevier Inc. All rights reserved.

Type of Journal (Indian or Foreign)Foreign
Impact Factor (IF)2.85
Divison category: 
Biochemical Sciences