Environment friendly crosslinked chitosan as a matrix for selective adsorption and purification of lipase of aspergillus niger
| Title | Environment friendly crosslinked chitosan as a matrix for selective adsorption and purification of lipase of aspergillus niger |
| Publication Type | Journal Article |
| Year of Publication | 2008 |
| Authors | Trimukhe, KD, Mahadik, ND, Gokhale, DV, Varma, A |
| Journal | International Journal of Biological Macromolecules |
| Volume | 43 |
| Issue | 5 |
| Pagination | 422-425 |
| Date Published | DEC |
| Type of Article | Article |
| ISSN | 0141-8130 |
| Keywords | Adsorption, Chitosan, Lipase, Matrix, Purification |
| Abstract | Chitosan and its derivatives have been used as affinity matrices for purification of lipase from Aspergillus niger NCIM 1207. Trimellitic anhydride (TMA)-crosslinked deacetylated chitin adsorbed lipase selectively, yielding approximately 5-fold purification of the crude lipase with 70% yield. Further 9-fold purification occurred on eluting through Sephacryl-100. These results suggest that chitosan derivatives can be used as inexpensive biopolymer matrices for the purification of lipases for industrial applications. (C) 2008 Elsevier B.V. All rights reserved. |
| DOI | 10.1016/j.ijbiomac.2008.08.005 |
| Type of Journal (Indian or Foreign) | Foreign |
| Impact Factor (IF) | 3.138 |
Divison category:
Biochemical Sciences
National Collection of Industrial Micr-organisms (NCIM)
Polymer Science & Engineering