01443nas a2200241 4500008004100000022001400041245013100055210006900186260007300255300001200328490000700340520057600347653001500923653001300938653001100951653001100962653001700973100002100990700002001011700002001031700002301051856012701074 2008 eng d a0141-813000aEnvironment friendly crosslinked chitosan as a matrix for selective adsorption and purification of lipase of aspergillus niger0 aEnvironment friendly crosslinked chitosan as a matrix for select aPO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSbELSEVIER SCIENCE BVcDEC a422-4250 v433 a
Chitosan and its derivatives have been used as affinity matrices for purification of lipase from Aspergillus niger NCIM 1207. Trimellitic anhydride (TMA)-crosslinked deacetylated chitin adsorbed lipase selectively, yielding approximately 5-fold purification of the crude lipase with 70% yield. Further 9-fold purification occurred on eluting through Sephacryl-100. These results suggest that chitosan derivatives can be used as inexpensive biopolymer matrices for the purification of lipases for industrial applications. (C) 2008 Elsevier B.V. All rights reserved.
10aAdsorption10aChitosan10aLipase10aMatrix10aPurification1 aTrimukhe, K., D.1 aMahadik, N., D.1 aGokhale, D., V.1 aVarma, Anjanikumar uhttp://library.ncl.res.in/content/environment-friendly-crosslinked-chitosan-matrix-selective-adsorption-and-purification-0