%0 Journal Article %J International Journal of Biological Macromolecules %D 2008 %T Environment friendly crosslinked chitosan as a matrix for selective adsorption and purification of lipase of aspergillus niger %A Trimukhe, K. D. %A Mahadik, N. D. %A Gokhale, D. V. %A Varma, Anjanikumar %K Adsorption %K Chitosan %K Lipase %K Matrix %K Purification %X

Chitosan and its derivatives have been used as affinity matrices for purification of lipase from Aspergillus niger NCIM 1207. Trimellitic anhydride (TMA)-crosslinked deacetylated chitin adsorbed lipase selectively, yielding approximately 5-fold purification of the crude lipase with 70% yield. Further 9-fold purification occurred on eluting through Sephacryl-100. These results suggest that chitosan derivatives can be used as inexpensive biopolymer matrices for the purification of lipases for industrial applications. (C) 2008 Elsevier B.V. All rights reserved.

%B International Journal of Biological Macromolecules %I ELSEVIER SCIENCE BV %C PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS %V 43 %P 422-425 %8 DEC %G eng %N 5 %9 Article %3

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%R 10.1016/j.ijbiomac.2008.08.005