Influence of hormones and medium components on expression of dipyranocoumarins in cell suspension cultures of calophyllum inophyllum L.
| Title | Influence of hormones and medium components on expression of dipyranocoumarins in cell suspension cultures of calophyllum inophyllum L. |
| Publication Type | Journal Article |
| Year of Publication | 2009 |
| Authors | Pawar, KD, Thengane, SRatnakar |
| Journal | Process Biochemistry |
| Volume | 44 |
| Issue | 8 |
| Pagination | 916-922 |
| Date Published | AUG |
| ISSN | 1359-5113 |
| Keywords | Calophyllum inophyllum, dipyranocoumarins, Expression pattern, HPLC, Medium components, Suspension culture |
| Abstract | Cell suspension cultures were initiated separately from leaf and nodal/internodal calluses for the study of influence of hormones and medium components on biomass growth and expression of dipyranocoumarins. Highest 6.2 times biomass was enhanced in suspension cultures of nodal/internodal callus supplemented with threefold total sulphate. Picloram 8.28 mu M along with BAP 8.88 mu M enhanced 295.05 times inophyllum A in suspension cultures of leaf callus whereas IBA 14.70 mu M along with BAP 4.44 mu M in suspension cultures of leaf callus enhanced 1065 times inophyllum B. IBA 4.90 mu M alone in suspension cultures of nodal/internodal callus enhanced maximum 616 times inophyllum C. Only IBA 9.80 mu M in suspension cultures of leaf callus enhanced 23.22 times inophyllum P. Variation in nitrate and sulphate had maximum positive influence on expression of inophyllums A and C and vitamins had maximum positive influence on expression of inophyllums A, C and B. (C) 2009 Elsevier Ltd. All rights reserved. |
| DOI | 10.1016/j.procbio.2009.03.005 |
| Type of Journal (Indian or Foreign) | Foreign |
| Impact Factor (IF) | 2.648 |