%0 Journal Article %J Process Biochemistry %D 2009 %T Influence of hormones and medium components on expression of dipyranocoumarins in cell suspension cultures of calophyllum inophyllum L. %A Pawar, Kiran D. %A Thengane, Shubhada Ratnakar %K Calophyllum inophyllum %K dipyranocoumarins %K Expression pattern %K HPLC %K Medium components %K Suspension culture %X

Cell suspension cultures were initiated separately from leaf and nodal/internodal calluses for the study of influence of hormones and medium components on biomass growth and expression of dipyranocoumarins. Highest 6.2 times biomass was enhanced in suspension cultures of nodal/internodal callus supplemented with threefold total sulphate. Picloram 8.28 mu M along with BAP 8.88 mu M enhanced 295.05 times inophyllum A in suspension cultures of leaf callus whereas IBA 14.70 mu M along with BAP 4.44 mu M in suspension cultures of leaf callus enhanced 1065 times inophyllum B. IBA 4.90 mu M alone in suspension cultures of nodal/internodal callus enhanced maximum 616 times inophyllum C. Only IBA 9.80 mu M in suspension cultures of leaf callus enhanced 23.22 times inophyllum P. Variation in nitrate and sulphate had maximum positive influence on expression of inophyllums A and C and vitamins had maximum positive influence on expression of inophyllums A, C and B. (C) 2009 Elsevier Ltd. All rights reserved.

%B Process Biochemistry %I ELSEVIER SCI LTD %C THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND %V 44 %P 916-922 %8 AUG %G eng %N 8 %3

Foreign

%4 2.648 %R 10.1016/j.procbio.2009.03.005