EGCG impedes human Tau aggregation and interacts with Tau

TitleEGCG impedes human Tau aggregation and interacts with Tau
Publication TypeJournal Article
Year of Publication2020
AuthorsSonawane, SKishor, Chidambaram, H, Boral, D, Gorantla, NVijay, Balmik, AAnkur, Dangi, A, Ramasamy, S, Marelli, UKiran, Chinnathambi, S
JournalScientific Reports
Volume10
Issue1
Pagination12579
Date PublishedJUL
Type of ArticleArticle
ISSN2045-2322
Abstract

Tau aggregation and accumulation is a key event in the pathogenesis of Alzheimer's disease. Inhibition of Tau aggregation is therefore a potential therapeutic strategy to ameliorate the disease. Phytochemicals are being highlighted as potential aggregation inhibitors. Epigallocatechin-3-gallate (EGCG) is an active phytochemical of green tea that has shown its potency against various diseases including aggregation inhibition of repeat Tau. The potency of EGCG in altering the PHF assembly of full-length human Tau has not been fully explored. By various biophysical and biochemical analyses like ThS fluorescence assay, MALDI-TOF analysis and Isothermal Titration Calorimetry, we demonstrate dual effect of EGCG on aggregation inhibition and disassembly of full-length Tau and their binding affinity. The IC50 for Tau aggregation by EGCG was found to be 64.2 mu M.

DOI10.1038/s41598-020-69429-6
Type of Journal (Indian or Foreign)

Foreign

Impact Factor (IF)

3.998

Divison category: 
Biochemical Sciences
Central NMR Facility
Organic Chemistry

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