TY - JOUR T1 - EGCG impedes human Tau aggregation and interacts with Tau JF - Scientific Reports Y1 - 2020 A1 - Sonawane, Shweta Kishor A1 - Chidambaram, Hariharakrishnan A1 - Boral, Debjyoti A1 - Gorantla, Nalini Vijay A1 - Balmik, Abhishek Ankur A1 - Dangi, Abha A1 - Ramasamy, Sureshkumar A1 - Marelli, Udaya Kiran A1 - Chinnathambi, Subashchandrabose AB -

Tau aggregation and accumulation is a key event in the pathogenesis of Alzheimer's disease. Inhibition of Tau aggregation is therefore a potential therapeutic strategy to ameliorate the disease. Phytochemicals are being highlighted as potential aggregation inhibitors. Epigallocatechin-3-gallate (EGCG) is an active phytochemical of green tea that has shown its potency against various diseases including aggregation inhibition of repeat Tau. The potency of EGCG in altering the PHF assembly of full-length human Tau has not been fully explored. By various biophysical and biochemical analyses like ThS fluorescence assay, MALDI-TOF analysis and Isothermal Titration Calorimetry, we demonstrate dual effect of EGCG on aggregation inhibition and disassembly of full-length Tau and their binding affinity. The IC50 for Tau aggregation by EGCG was found to be 64.2 mu M.

VL - 10 IS - 1 U3 -

Foreign

U4 -

3.998

ER -