Acetone and methanol fruit extracts of terminalia paniculata inhibit HIV-1 infection in vitro
| Title | Acetone and methanol fruit extracts of terminalia paniculata inhibit HIV-1 infection in vitro |
| Publication Type | Journal Article |
| Year of Publication | 2017 |
| Authors | Durge, A, Jadaun, P, Wadhwani, A, Chinchansure, AA, Said, M, Thulasiram, HV, Joshi, SP, Kulkarni, SS |
| Journal | Natural Product Research |
| Volume | 31 |
| Issue | 12 |
| Pagination | 1468-1471 |
| Date Published | JAN |
| Abstract | In this study, we report the in vitro anti-HIV1 activity of acetone and methanol extracts of fruit of Terminalia paniculata. Cytotoxicity tests were conducted on TZM-bl cells and peripheral blood mononuclear cells (PBMC), the CC50 values of both the extracts were 260g/mL. Using TZM-bl cells, the extracts were tested for their ability to inhibit replication of two primary isolates HIV-1 (X4, Subtype D) and HIV-1 (R5, Subtype C). The activity against HIV-1 primary isolate (R5, Subtype C) was confirmed using activated PBMC and by quantification of HIV-1 p24 antigen. Both the extracts showed anti-HIV1 activity in a dose-dependent manner. The EC50 values of the acetone and methanol extracts of T. paniculata were 10.3g/mL. The enzymatic assays were performed to determine the mechanism of action which indicated that the anti-HIV1 activity might be due to inhibition of reverse transcriptase (77.7% inhibition) and protease (69.9% inhibition) enzymes. |
| DOI | 10.1080/14786419.2016.1258561 |
| Type of Journal (Indian or Foreign) | Foreign |
| Impact Factor (IF) | 1.057 |
Divison category:
Organic Chemistry