Hyperthermia and doxorubicin release by Fol-LSMO nanoparticles induce apoptosis and autophagy in breast cancer cells
| Title | Hyperthermia and doxorubicin release by Fol-LSMO nanoparticles induce apoptosis and autophagy in breast cancer cells |
| Publication Type | Journal Article |
| Year of Publication | 2022 |
| Authors | Kulkarni-Dwivedi, N, Patel, PR, V. Shravage, B, Umrani, RD, Paknikar, KM, Jadhav, SH |
| Journal | Nanomedicine |
| Volume | 17 |
| Issue | 25 |
| Pagination | 1929-1949 |
| Date Published | OCT |
| Type of Article | Article |
| ISSN | 1743-5889 |
| Keywords | Apoptosis, Autophagy, Breast cancer, combination therapy, doxorubicin, Folic acid, hyperthermia, LSMO, MDA-MB231 cells, PEG |
| Abstract | Background: Studies on the anticancer effects of lanthanum strontium manganese oxide (LSMO) nanoparticles (NPs)-mediated hyperthermia at cellular and molecular levels are scarce. Materials & methods: LSMO NPs conjugated with folic acid (Fol-LSMO NPs) were synthesized, followed by doxorubicin-loading (DoxFol-LSMO NPs), and their effects on breast cancer cells were investigated. Results: Hyperthermia (45 degrees C) and combination treatments exhibited the highest (similar to 95%) anticancer activity with increased oxidative stress. The involvement of intrinsic mitochondria-mediated apoptotic pathway and induction of autophagy was noted. Cellular and molecular evidence confirmed the crosstalk between apoptosis and autophagy, involving Beclin1, Bcl2 and Caspase-3 genes with free reactive oxygen species presence. Conclusion: The study confirmed hyperthermia and doxorubicin release by Fol-LSMO NPs induces apoptosis and autophagy in breast cancer cells. |
| DOI | 10.2217/nnm-2022-0171 |
| Type of Journal (Indian or Foreign) | Foreign |
| Impact Factor (IF) | 6.096 |