Superior processability of Antheraea mylitta silk with cryo-milling: performance in bone tissue regeneration

TitleSuperior processability of Antheraea mylitta silk with cryo-milling: performance in bone tissue regeneration
Publication TypeJournal Article
Year of Publication2022
AuthorsParekh, N, Bijosh, CK, Kane, K, Panicker, A, Nisal, A, Wangikar, P, Agawane, S
JournalInternational Journal of Biological Macromolecules
Volume213
Pagination155-165
Date PublishedJUL
Type of ArticleArticle
ISSN0141-8130
KeywordsBone tissue engineering, Calvarial defect, hMSCs, Non-mulberry silk fiber, Processing technique
Abstract

Non-mulberry silk polymers have a promising future in biomedical applications. However, the dissolution of nonmulberry silk fiber is a still challenge and this poor processability has limited the use of this material. Here, we report a unique protocol to process the Antheraea mylitta (AM) silk fiber. We have shown that the cryo-milling of silk fiber reduces the beta sheet content by more than 10% and results in an SF powder that completely dissolves in routine solvents like trifluoroacetic acid (TFA) within few hours to form highly concentrated solutions (\~20 wt %). Further, these solutions can be processed using conventional processing techniques such as electrospinning to form 3D scaffolds. Bombyx mori (BM) silk was used as a control sample in the study. In-vitro studies were also performed to monitor cell adhesion and proliferation and hMSCs differentiation into osteogenic lineage. Finally, the osteogenic potential of the scaffolds was also evaluated by a 4-week implantation study in rat calvarial model. The in-vitro and in-vivo results show that the processing techniques do not affect the biocompatibility of the material and the AM scaffolds support bone regeneration. Our results, thus, show that cryo-milling facilitates enhanced processability of non-mulberry silk and therefore expands its potential in biomedical applications.

DOI10.1016/j.ijbiomac.2022.05.122
Type of Journal (Indian or Foreign)

Foreign

Impact Factor (IF)

8.025

Divison category: 
Biochemical Sciences
Polymer Science & Engineering
Database: 
Web of Science (WoS)

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