Simultaneous determination of anthraquinone, flavonoids, and phenolic antidiabetic compounds from cassia auriculata seeds by validated UHPLC based MS/MS method
| Title | Simultaneous determination of anthraquinone, flavonoids, and phenolic antidiabetic compounds from cassia auriculata seeds by validated UHPLC based MS/MS method |
| Publication Type | Journal Article |
| Year of Publication | 2020 |
| Authors | Girme, A, Saste, G, Chinchansure, A, Joshi, S, Kunkulol, R, Hingorani, L, Patwardhan, B |
| Journal | Mass Spectrometry Letters |
| Volume | 11 |
| Issue | 4 |
| Pagination | 82-89 |
| Date Published | DEC |
| Type of Article | Article |
| Abstract | A systematic isolation and characterization study for Cassia auriculata (CA) seeds resulted in identifying antidiabetic compounds 1,3,8-trihydroxyanthraquinone and quercetin, quercetin-3-O-rutinoside, gallic acid, caffeic acid, ferulic acid, and ellagic acid. The ultra-high-performance liquid chromatography based triple quadrupole mass spectrometry methodology was developed and validated for simultaneous identification and confirmation of these compounds from CA seeds. Multiple reaction monitoring (MRM) based quantification method was developed with MRM optimizer software for MS1 and MS2 mass analysis. The method was optimized on precursor ions and product ions with the ion ratio of each compound. The calibration curves of seven bioactive analytes showed excellent linearity (r2 ≥ 0.99). The quantitation results found precise (RSD, |
| DOI | 10.5478/MSL.2020.11.4.82 |
| Type of Journal (Indian or Foreign) | Foreign |
| Impact Factor (IF) | 0.222 |
Divison category:
Organic Chemistry
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