Silver nanoparticles were synthesized by continuous flow methods using biosurfactants, namely, oleic acid sophorolipid (OASL) and stearic acid sophorolipid (SASL). Both the sophorolipids can act as reducing and capping agents. The effect of temperature on the completion of nanoparticle formation and the particle growth dynamics (size) were studied in batch mode. While the completion of the reaction using oleic acid sophorolipid needed 20 min, only 5 min were required with the stearic acid sophorolipid as capping and reducing agent. Hence all the continuous flow experiments were carried out using the stearic acid sophorolipid. The continuous flow synthesis of silver nanoparticles was carried out in a stainless steel helical coil and also in a spiral polymeric minichannel reactor. The DLS results show that higher flow rate leads to the formation of bigger and polydisperse particles because of incomplete reactions. Higher residence time allowed the completion of reaction leading to spherical, small and monodisperse particles.