Biogenic synthesis of silver nanoparticles using nicotiana tobaccum leaf extract and study of their antibacterial effect
| Title | Biogenic synthesis of silver nanoparticles using nicotiana tobaccum leaf extract and study of their antibacterial effect |
| Publication Type | Journal Article |
| Year of Publication | 2011 |
| Authors | Prasad, KSuranjit, Pathak, D, Patel, A, Dalwadi, P, Prasad, R, Patel, P, Selvaraj, K |
| Journal | African Journal of Biotechnology |
| Volume | 10 |
| Issue | 41 |
| Pagination | 8122-8130 |
| Date Published | AUG |
| ISSN | 1684-5315 |
| Keywords | FT-IR spectroscopy, photoluminescence, silver nanoparticles, Tobacco, UV-Vis absorption |
| Abstract | A green synthesis of silver nanoparticle was carried out using tobacco leaf extract. Synthesized nanoparticles were characterized using UV-Vis absorption spectroscopy, TEM, EDAX, FT-IR and photoluminescence study, respectively. UV-Vis absorption spectroscopy of prepared silver colloidal solution showed absorption maxima at 418 nm. Excitation maximum and emission maximum obtained from photoluminescence study were found at 414 and 576 nm, respectively. TEM analysis showed average particle size of 8 nm, while SAED pattern confirmed the crystalline nature of synthesized nanoparticles. FT-IR analysis indicated the involvement of carboxyl (-C = O), hydroxyl (-OH) and amine (-NH) functional groups of tobacco leaf extract in preparation of silver nanoparticles. EDAX analysis showed proportion of silver (54.55%) among other elements in nanoparticle. Pseudomonas aeruginosa and Escherichia coli DH5 alpha showed highest sensitivity towards silver nanoparticles. |
| Type of Journal (Indian or Foreign) | Foreign |
| Impact Factor (IF) | 0.655 |