The catalytic efficacy of (1R,2R)-bis[(S)-prolinamido]cyclohexane 1, prepared from the readily available natural amino acid L-proline has been studied for the direct asymmetric aldol reaction of cyclohexanone with substituted benzaldehydes at room temperature in presence of various acid additives. A wide variety of acids e.g. aliphatic fatty acid, chiral acid, sulphonic acid, aromatic acid, etc. have been used as additive for the aldol reaction in aqueous media. A loading of 10 mol% of catalyst 1 is employed in this reaction, and good yields (up to 98% yield), diastereoselectivity (up to 96% de) and enantioselectivities (up to 87% ee) can be achieved in aqueous media within very short reaction time (1-4 hours).