Studies on optimization of cultural conditions and effect of media constituents on production of intracellular penicillin V acylase from Erwinia aroideae (DSMZ 30186)
Title | Studies on optimization of cultural conditions and effect of media constituents on production of intracellular penicillin V acylase from Erwinia aroideae (DSMZ 30186) |
Publication Type | Journal Article |
Year of Publication | 2008 |
Authors | Atul, K, Vivek, L, Archana, P |
Journal | Research Journal of Biotechnology |
Volume | 3 |
Issue | 2 |
Pagination | 26-30 |
Date Published | MAY |
ISSN | 0973-6263 |
Keywords | carbon sources, Erwinia aroideae, intracellular, nitrogen sources, Penicillin V acylase |
Abstract | Penicillin V acylase (PVA) is a pharmaceutically important enzyme. Erwinia aroideae (DSMZ 30186) produced high levels of intracellular penicillin V acylase. Amongst various carbon sources used (2.0 %); fructose, galactose, sucrose and mannitol increased enzyme production up to 579, 544, 534 and 504 IU/g dry weight (DW), respectively, compared to minimal media (263 IU/g DW); however maximum PVA productivity (2796 IU/ L) was achieved using fructose. Ammonium ions escalated the enzyme production to 1266.9 IU/g DW; however sodium glutamate was the best nitrogen source for overall productivity of enzyme (2045 IU/ L). Cornsteep liquor and skim milk were used as supplements; 1.0 % cornsteep liquor and 3.0 % skim milk were optimum for the production of PVA up to 823 and 93 IU/g DW, respectively. Optimum cultural conditions for the production of PVA from E. aroideae were standardized. Erwinia aroideae produced 1543 IU/g DW penicillin V acylase in Erlenmeyer flasks (250 ml) containing 50 ml of minimal medium with 0.3% ammonium sulphate, pH 7. 0 at 28 degrees C and 180 rpm when incubated for 56 hr. |
Type of Journal (Indian or Foreign) | Indian |
Impact Factor (IF) | 0.284 |