In situ pH maintenance for mammalian cell cultures in shake flasks and tissue culture flasks
| Title | In situ pH maintenance for mammalian cell cultures in shake flasks and tissue culture flasks |
| Publication Type | Journal Article |
| Year of Publication | 2012 |
| Authors | Pradhan, K, Pant, T, Gadgil, M |
| Journal | Biotechnology Progress |
| Volume | 28 |
| Issue | 6 |
| Pagination | 1605-1610 |
| Date Published | NOV |
| ISSN | 8756-7938 |
| Keywords | high throughput screening, pH control for cell culture, pH control in shake flask, pH control in tissue culture flasks, small scale platforms for cell culture |
| Abstract | pH in animal cell cultures decreases due to production of metabolites like lactate. pH control via measurement and base addition is not easily possible in small-scale culture formats like tissue-culture flasks and shake flasks. A hydrogel-based system is reported for in situ pH maintenance without pH measurement in such formats, and is demonstrated to maintain pH between 6.8 and 7.2 for a suspension CHO cell line in CD CHO medium and between 7.3 and 7.5 for adherent A549 cells in DMEM:F12 containing 10% FBS. This system for pH maintenance, along with our previous report of hydrogels for controlled nutrient delivery in shake flasks can allow shake flasks to better mimic bioreactor-based fed batch operation for initial screening during cell line and process development for recombinant protein production in mammalian cells. (C) 2012 American Institute of Chemical Engineers Biotechnol. Prog., 2012 |
| DOI | 10.1002/btpr.1614 |
| Type of Journal (Indian or Foreign) | Foreign |
| Impact Factor (IF) | 1.853 |