Silk fibroin micro-particle scaffolds with superior compression modulus and slow bioresorption for effective bone regeneration
| Title | Silk fibroin micro-particle scaffolds with superior compression modulus and slow bioresorption for effective bone regeneration |
| Publication Type | Journal Article |
| Year of Publication | 2018 |
| Authors | Nisal, A, Sayyad, R, Dhavale, P, Khude, B, Deshpande, R, Mapare, V, Shukla, S, Venugopalan, P |
| Journal | Scientific Reports |
| Volume | 8 |
| Pagination | Article Number: 7235 |
| Date Published | MAY |
| Abstract | Silk fibroin (SF), a natural polymer produced by Bombyx mori silkworms, has been extensively explored to prepare porous scaffolds for tissue engineering applications. Here, we demonstrate, a scaffold made of SF, which exhibits compression modulus comparable to natural cancellous bone while retaining the appropriate porosities and interconnected pore architecture. The scaffolds also exhibit high resistance to in-vitro proteolytic degradation due to the dominant beta sheet conformation of the SF protein. Additionally, the scaffolds are prepared using a simple method of microparticle aggregation. We also demonstrate, for the first time, a method to prepare SF micro-particles using a Hexafluoroisopropanol-Methanol solvent-coagulant combination. SF microparticles obtained using this method are monodisperse, spherical, non-porous and extremely crystalline. These micro-particles have been further aggregated together to form a 3D scaffold. The aggregation is achieved by random packing of these microparticles and fusing them together using a dilute SF solution. Preliminary in-vitro cell culture and in-vivo implantation studies demonstrate that the scaffolds are biocompatible and they exhibit the appropriate early markers, making them promising candidates for bone regeneration. |
| DOI | 10.1038/s41598-018-25643-x |
| Type of Journal (Indian or Foreign) | Foreign |
| Impact Factor (IF) | 4.259 |
Divison category:
Polymer Science & Engineering
Add new comment