Silk degumming and utilization of silk sericin by hydrolysis using alkaline protease from beauveria Sp. (MTCC 5184): a green approach
| Title | Silk degumming and utilization of silk sericin by hydrolysis using alkaline protease from beauveria Sp. (MTCC 5184): a green approach |
| Publication Type | Journal Article |
| Year of Publication | 2017 |
| Authors | More, SV, Chavan, S, Prabhune, AAshutosh |
| Journal | Journal of Natural Fibers |
| Volume | 15 |
| Issue | 3 |
| Pagination | 1-11 |
| Date Published | JUL |
| Type of Article | Article |
| ISSN | 1544-0478 |
| Keywords | Alkaline Protease, bivoltine, degumming, Silk, weight loss |
| Abstract | Conventionally, degumming is carried out at 90°C--110°C temperature by boiling the raw silk with Marseilles soap and sodium bicarbonate which eventually requires a lot of water and energy. In this study, degumming of Chinese bivoltine raw silk fibres with alkaline protease produced by Beauveria sp. (MTCC 5184) is studied. Complete degumming was obtained in 45 min with 75 units of enzyme per gram of silk. Degumming was found to be optimal at 50°C and pH 9.0. Scanning electron micrographs showed that the sericin deposits were removed and the obtained fibres were clean, separated, had smooth feel with shine as compared to untreated fibres. Sericin isolated from silk cocoon (by-product which goes waste) was hydrolyzed with the same alkaline protease obtained from Beauveria sp. to get small molecular weight peptides. These peptides can be utilized further for cosmetic, pharmaceutical, and various industrial applications. |
| DOI | 10.1080/15440478.2017.1330718 |
| Type of Journal (Indian or Foreign) | Foreign |
| Impact Factor (IF) | 0.582 |
Divison category:
Biochemical Sciences
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