Quantitative, automated, high content screening assay approach to anti-angiogenicdrug discovery

TitleQuantitative, automated, high content screening assay approach to anti-angiogenicdrug discovery
Publication TypeConference Paper
Year of Publication2015
AuthorsNawale, L, Sarkar, D
Conference NameNational Science Day, At CSIR-NCL
Date PublishedFEB
Conference LocationCombi Chem-Bio Resource Center, OCD, National Chemical Laboratory, Pune - 411 008
Abstract

Angiogenesisenhancescancer,maculardegeneration,etc.. Currentangiogenicassays--indirect,manual,timeconsuming,laborintensive,difficulttoquantify,andexpensive. Wedevelopedanautomated,quantitative,HighContentScreeningangiogenictubeformationassaywouldbenefitdrugdiscoveryforseveraltherapeuticareas. Humanendothelialcellswereseededwithpro-oranti-angiogeniccompoundsonaMatrigelTMlayerina96-wellplate.Afterangiogenictubesformation,thepositivecontrolsandthetestsampleswerefixed,fluorescentlylabeled,automaticallyimagedonCellomics’ArrayScan®HCSReader,andquantitativelyanalyzedon-the-flybytheTubeFormationBioApplicationsoftwaremodule. Multiplefeaturesrelevantforangiogenesisandangiogenictubeformationwereautomaticallymeasured. Thisrobustandreliableassaythusenabledthelarge-scale,quantitative,screeningandcharacterizationofcompoundsaffectingbothpro-andanti-angiogenicpathways.
Divison category: 
Biochemical Sciences