Production, purification, and characterization of taxol and 10-DABIII from a new endophytic fungus gliocladium sp isolated from the Indian yew tree, taxus baccata
| Title | Production, purification, and characterization of taxol and 10-DABIII from a new endophytic fungus gliocladium sp isolated from the Indian yew tree, taxus baccata |
| Publication Type | Journal Article |
| Year of Publication | 2009 |
| Authors | Sreekanth, D, Syed, A, Sarkar, S, Sarkar, D, Santhakumari, B, Ahmad, A, Khan, MIslam |
| Journal | Journal of Microbiology and Biotechnology |
| Volume | 19 |
| Issue | 11 |
| Pagination | 1342-1347 |
| Date Published | NOV |
| ISSN | 1017-7825 |
| Keywords | 10 DAB III, Endophytic fungi, Gliocladium sp., Taxol, Taxus baccata |
| Abstract | We have isolated endophytic fungi from the Indian yew tree, Taxus baccata, and then screened for taxol production. Out of the 40 fungal cultures screened, one fungus Gliocladium sp. was found to produce taxol and 10-DABIII (10-deacetyl baccatin III). These compounds were purified by TLC and HPLC and characterized using UV-spectroscopy, ESI-MS, MS/MS, and proton NMR. One liter of Gliocladium sp. culture yielded 10 mu g of taxol and 65 mu g of 10-DABIII. The purified taxol from the fungus showed cytotoxicity towards cancer lines HL-60 (leukemia), A431 (epidermal carcinoma), and MCF-7 (breast cancer). |
| DOI | 10.4014/jmb.0904.04041 |
| Type of Journal (Indian or Foreign) | Foreign |
| Impact Factor (IF) | 1.224 |
Divison category:
Biochemical Sciences
Center for Material Characterization (CMC)
Organic Chemistry