Production and single step purification of cyclodextrin glycosyltransferase from alkalophilic bacillus firmus by ion exchange chromatography
| Title | Production and single step purification of cyclodextrin glycosyltransferase from alkalophilic bacillus firmus by ion exchange chromatography |
| Publication Type | Journal Article |
| Year of Publication | 2008 |
| Authors | Savergave, LS, Mule, SS, Jogdand, VV, Nene, SN, Gadre, RV |
| Journal | Biochemical Engineering Journal |
| Volume | 39 |
| Issue | 3 |
| Pagination | 510-515 |
| Date Published | MAY |
| ISSN | 1369-703X |
| Keywords | alkalophilic, Bacillus firmus, CGTase, cyclodextrin, Purification |
| Abstract | Production and purification of starch digesting cyclodextrin glycosyl transferase (CGTase) from alkalophilic Bacillus firmus was investigated. Fermentation was carried out in 141 bioreactor at 28 degrees C using a medium containing dextrin, yeast extract, peptone, (NH4)H2PO4 and MgSO4.7H(2)O. The extracellular enzyme was concentrated by tangential flow ultrafiltration. The concentrated enzyme was chromatographed using DEAE-sepharose and phenyl sepharose. DEAE-sepharose could be used to purify CGTase in a single step with 23.1 fold purification and 80.6% recovery. The enzyme obtained had homogeneity and the molecular weight was 76 kDa confirmed by SDS-PAGE. (c) 2007 Elsevier B.V. All rights reserved. |
| DOI | 10.1016/j.bej.2007.09.020 |
| Type of Journal (Indian or Foreign) | Foreign |
| Impact Factor (IF) | 2.692 |
Divison category:
Chemical Engineering & Process Development