Poly(o-anisidine) films on mild steel: electrochemical synthesis and biosensor application

TitlePoly(o-anisidine) films on mild steel: electrochemical synthesis and biosensor application
Publication TypeJournal Article
Year of Publication2007
AuthorsPatil, D, Gaikwad, AB, Patil, P
JournalJournal of Physics D-Applied Physics
Volume40
Issue8
Pagination2555-2562
Date PublishedAPR
Type of ArticleArticle
ISSN0022-3727
Abstract

Poly(o-anisidine) (POA) films were synthesized on mild steel from an aqueous oxalic acid solution by electrochemical polymerization of o-anisidine using cyclic voltammetry. These films were characterized by cyclic voltammetry, UV-visible absorption spectroscopy, Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM). The enzyme glucose oxidase (GOx) was entrapped into the POA film by a physical adsorption method. The resulting POA-GOx films were characterized by UV-visible absorption spectroscopy, FTIR and SEM. The amperometric response of the POA-GOx films was measured as a function of glucose concentration in phosphate buffer solution (pH 7.3). The POA-GOx films exhibited a fast amperometric response (1-5 s) and a linear response in the range of 2-20 mM glucose. The maximum current density and Michaelis-Menten constant of POA/GOx films were found to be similar to 406 mu A cm(-2) and 1.03 mM, respectively. The shelf stability, operational stability and thermal stability of these films were also investigated.

DOI10.1088/0022-3727/40/8/021
Type of Journal (Indian or Foreign)

Foreign

Impact Factor (IF)2.772
Divison category: 
Center for Material Characterization (CMC)