Poly(d,l-lactide-<i>co</i>-glycolide) surface-anchored biotin-loaded irinotecan nanoparticles for active targeting of colon cancer

TitlePoly(d,l-lactide-co-glycolide) surface-anchored biotin-loaded irinotecan nanoparticles for active targeting of colon cancer
Publication TypeJournal Article
Year of Publication2024
AuthorsGiram, PS, Nimma, R, Bulbule, A, Yadav, ASingh, Gorain, M, Radharani, NNaga Venka, Kundu, GC, Garnaik, B
JournalACS Omega
Volume9
Issue3
Pagination3807-3826
Date PublishedJAN
Type of ArticleArticle
ISSN2470-1343
Abstract

A poly-(d,l-lactide-co-glycolide) (PLGA) copolymer was synthesized using the ring-opening polymerization of d,l-lactide and glycolide monomers in the presence of zinc proline complex in bulk through the green route and was well characterized using attenuated total reflectance-Fourier transform infrared, H-1 and C-13 nuclear magnetic resonance, gel permeation chromatography, differential scanning calorimetry, X-ray diffraction, matrix-assisted laser desorption/ionization time-of-flight, etc. Furthermore, PLGA-conjugated biotin (PLGA-B) was synthesized using the synthesized PLGA and was employed to fabricate nanoparticles for irinotecan (Ir) delivery. These nanoparticles (PLGA-NP-Ir and PLGA-B-NP-Ir) were tested for physicochemical and biological characteristics. PLGA-B-NP-Ir exhibited a stronger cellular uptake and anticancer activity as compared to PLGA-NP-Ir in CT-26 cancer cells (log p < 0.05). The accumulation and retention of fluorescence-labeled nanoparticles were observed to be better in CT-26-inoculated solid tumors in Balb/c mice. The PLGA-B-NP-Ir-treated group inhibited tumor growth significantly more (log p < 0.001) than the untreated control, PLGA-NP-Ir, and Ir-treated groups. Furthermore, no body weight loss, hematological, and blood biochemical tests demonstrated the nanocarriers' nontoxic nature. This work presents the use of safe PLGA and the demonstration of a proof-of-concept of biotin surface attached PLGA nanoparticle-mediated active targeted Ir administration to combat colon cancer. To treat colon cancer, PLGA-B-NP-Ir performed better due to specific active tumor targeting and greater cellular uptake due to biotin.

DOI10.1021/acsomega.3c07833
Type of Journal (Indian or Foreign)

Foreign

Impact Factor (IF)

4.1

Divison category: 
Polymer Science & Engineering
Database: 
Web of Science (WoS)

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