Molecular methods for identification of clostridium tetani by targeting neurotoxin
| Title | Molecular methods for identification of clostridium tetani by targeting neurotoxin |
| Publication Type | Book Chapter |
| Year of Publication | 2017 |
| Authors | Dharne, MS, Nagoba, BS, Gohil, KN |
| Book Title | Methods Molecular Biology |
| Volume | 1600 |
| Pagination | 37-47 |
| Abstract | Tetanus is a potentially fatal muscle spasm disease. It is an important public health problem, especially in rural/tribal areas of developing countries. Tetanus toxin, a neurotoxin (tetanospasmin ), is the most important virulence factor that plays a key role in the pathogenicity of tetanus . Confirmation of virulence by confirming the production of tetanospasmin by infecting species forms the most important part in the diagnosis of tetanus . Various molecular methods have been devised for confirmation of diagnosis by targeting different genes. The most common molecular methods are tetanospasmin producing (TetX) gene-targeted methods using TetX-specific primers. Here, we describe various molecular methods targeting TetX gene such as polymerase chain reaction, pulsed-field gel electrophoresis, Southern blotting, loop-mediated isothermal amplification assay, etc. to confirm the virulence of Cl. tetani. |
| DOI | 10.1007/978-1-4939-6958-6_4 |
Divison category:
Biochemical Sciences
National Collection of Industrial Micr-organisms (NCIM)