Molecular Fe-complex as a catalyst probe for in-gel visual detection of proteins via signal amplification
| Title | Molecular Fe-complex as a catalyst probe for in-gel visual detection of proteins via signal amplification |
| Publication Type | Journal Article |
| Year of Publication | 2015 |
| Authors | Kumari, S, Panda, C, Mazumdar, S, Gupta, SSen |
| Journal | Chemical Communications |
| Volume | 51 |
| Issue | 83 |
| Pagination | 15257-15260 |
| Date Published | JUL |
| Type of Article | Article |
| ISSN | 1359-7345 |
| Abstract | We report the use of a molecular peroxidase mimic biuret-Fe-TAML for chemoselective labeling of proteins and the subsequent visual detection (<0.1 pmoles) of the conjugate in a polyacrylamide gel by catalytic signal amplification. Use of this probe in activity based protein profiling (ABPP) of serine proteases is also demonstrated. |
| DOI | 10.1039/c5cc04399a |
| Type of Journal (Indian or Foreign) | Foreign |
| Impact Factor (IF) | 6.567 |
Divison category:
Chemical Engineering & Process Development