Mannitol production from glycerol by resting cells of Candida magnoliae

TitleMannitol production from glycerol by resting cells of Candida magnoliae
Publication TypeJournal Article
Year of Publication2009
AuthorsKhan, A, Bhide, AJ, Gadre, RV
JournalBioresource Technology
Volume100
Issue20
Pagination4911-4913
Date PublishedOCT
ISSN0960-8524
KeywordsCandida magnoliae, Glycerol, Mannitol production, Resting cells
Abstract

Production of mannitol from glycerol by resting cells of Candida magnoliae under aerobic condition was investigated. The resting cells were Suspended in aqueous Solution of glycerol in Erlenmeyer flasks and incubated on rotary shaker. The samples were analyzed by ion exclusion-HPLC equipped with refractive index and UV detector. The resting cells of C. magnoliae produced mannitol from fructose, Sucrose and glycerol but not from glucose. Addition of yeast extract and/or potassium phosphate to the glycerol solution adversely affected its conversion to mannitol. The conversion of glycerol to mannitol was dependent on oxygen availability. Using testing cells, the yield of mannitol was as high as 45%. This is probably the first report of conversion of glycerol to mannitol by osmophilic yeast. (C) 2009 Elsevier Ltd. All rights reserved.

DOI10.1016/j.biortech.2009.04.048
Type of Journal (Indian or Foreign)Foreign
Impact Factor (IF)4.365
Divison category: 
Chemical Engineering & Process Development