Isolation and structural characterization of exopolysaccharide from marine Bacillus sp. and its optimization by Microbioreactor
| Title | Isolation and structural characterization of exopolysaccharide from marine Bacillus sp. and its optimization by Microbioreactor |
| Publication Type | Journal Article |
| Year of Publication | 2022 |
| Authors | Wagh, VS, Said, MS, Bennale, JS, Dastager, SG |
| Journal | Carbohydrate Polymers |
| Volume | 285 |
| Pagination | 119241 |
| Date Published | JUN |
| Type of Article | Article |
| ISSN | 0144-8617 |
| Keywords | EPS, marine, Microbioreactor, Optimization, structure |
| Abstract | In the present study, a cost-effective, robust Microbioreactor based production optimization of levan like exopolysaccharide from marine Bacillus sp. SGD-03 was analysed. FE-SEM analysis has showed the significant fibrillar structure of EPS. Size exclusion chromatography and other analytical data revealed that, produced EPS has a molecular weight of 1.0 x 10(4) Da and is composed of fructose monosaccharide with hydroxyl, carbonyl, and ether groups. The backbone structure of EPS has a branching pattern of beta-(2,6) linkages which confirms the similarity with available levan like polymers. The cost-effective media composition for levan production was demonstrated. The maximum yield of crude levan obtained was 123.9 g/L by response surface methodology using robust BioLector Pro Microbioreactor, and same has been validated with shake flask, 1 L and 10 L pilot-scale fermentation. |
| DOI | 10.1016/j.carbpol.2022.119241 |
| Type of Journal (Indian or Foreign) | Foreign |
| Impact Factor (IF) | 10.723 |