Direct somatic embryogenesis and plant regeneration in Garcinia indica Choiss
Title | Direct somatic embryogenesis and plant regeneration in Garcinia indica Choiss |
Publication Type | Journal Article |
Year of Publication | 2006 |
Authors | Thengane, SRatnakar, Deodhar, SR, Bhosle, SV, Rawal, SK |
Journal | Current Science |
Volume | 91 |
Issue | 8 |
Pagination | 1074-1078 |
Date Published | OCT |
Type of Article | Article |
ISSN | 0011-3891 |
Keywords | Garcinia indica, guttifereae, seeds, somatic embryos |
Abstract | Direct somatic embryogenesis without an intervening callus phase was induced from immature seeds of Gar- cinia indica Choiss. Woody plant medium supplemented with 6-benzyl amino purine (BAP) (4.44-22.19 mu m) alone or in combination with a-naphthaleneacetic acid (2.69 mu m) produces somatic embryos within a period of 2-3 weeks with 80% frequency. Embryo induction was observed all over the explant surface. Origin of the embryo was confirmed histologically from sub-epidermal layer of the seed. Maturation of these embryos was achieved after 12 weeks of culture on a medium containing BAP (16.08 mu m) in combination with indole-3-acetic acid (2.85-5.71 mu m) and/or kinetin (4.65 mu m). About 75% of the regenerated somatic embryos germinated into complete plantlets. The plantlets were acclimatized successfully with 92% survival in greenhouse. |
Type of Journal (Indian or Foreign) | Indian |
Impact Factor (IF) | 0.967 |