Differential protease activity augments polyphagy in helicoverpa armigera

TitleDifferential protease activity augments polyphagy in helicoverpa armigera
Publication TypeJournal Article
Year of Publication2013
AuthorsChikate, YR, Tamhane, VA, Joshi, RS, Gupta, VS, Giri, AP
JournalInsect Molecular Biology
Date PublishedJUN
Keywordsadaptation, chymotrypsin, Helicoverpa armigera, polyphagy, Trypsin

Helicoverpa armigera (Lepidoptera: Noctuidae) and other polyphagous agricultural pests are extending their plant host range and emerging as serious agents in restraining crop productivity. Dynamic regulation, coupled with a diversity of digestive and detoxifying enzymes, play a crucial role in the adaptation of polyphagous insects. To investigate the functional intricacy of serine proteases in the development and polyphagy of H.armigera, we profiled the expression of eight trypsin-like and four chymotrypsin-like phylogenetically diverse mRNAs from different life stages of H.armigera reared on nutritionally distinct host plants. These analyses revealed diet- and stage-specific protease expression patterns. The trypsins expressed showed structural variations, which might result in differential substrate specificity and interaction with inhibitors. Protease profiles in the presence of inhibitors and their mass spectrometric analyses revealed insight into their differential activity. These findings emphasize the differential expression of serine proteases and their consequences for digestive physiology in promoting polyphagy in H.armigera.

Funding Agency

Council of Scientific & Industrial Research (CSIR) - India

Type of Journal (Indian or Foreign)


Impact Factor (IF)


Divison category: 
Biochemical Sciences