Diastase induced green synthesis of bilayered reduced graphene oxide and its decoration with gold nanoparticles

TitleDiastase induced green synthesis of bilayered reduced graphene oxide and its decoration with gold nanoparticles
Publication TypeJournal Article
Year of Publication2017
AuthorsMaddinedi, SBabu, Mandal, BKumar, Patil, SHindurao, Andhalkar, VVilas, Ranjan, S, Dasgupta, N
JournalJournal of Photochemistry and Photobiology B-Biology
Volume166
Pagination252-258
Date PublishedJAN
AbstractIn this paper, we report an enzyme dependent, green one-pot deoxygenation cum decoration method to synthesize diastase-conjugated reduced graphene oxide (DRG) nanosheets, DRG/gold nanoparticles (DRG/Au) composite. The DRG synthesis was completed in 7 h under heating at 90 degrees C on water bath. Selected area electron diffraction (SAED) and Atomic force microscopy (AFM) study has revealed the formation of bilayered reduced graphene oxide sheets. Transmission electron microscopy (TEM) images of DRG/Au composite have shown the uniform decoration of gold nanoparticles (AuNPs) onto the DRG nanosheet surface. Fourier transform infrared spectroscopy (FTIR) and Raman results additionally have shown the functionalization of enzyme molecules onto the DRG nanosheet surface after reduction making it as an effective platform towards the efficient binding of gold nanoparticles. In vitro cytotoxicity studies by MIT assay on A549 and HCT116 cell lines exhibited that the cytotoxicity of the prepared graphene oxide (GO), DRG and DRG/Au is dose dependant. These results have shown that this synthetic method is effective for the production of large scale graphene in a low cost, simple and green method. Since this process avoids the use of hazardous and toxic substances, the produced DRG/Au composites are likely to offer various potential applications in biology and medicine. (C) 2016 Published by Elsevier B.V
DOI10.1016/j.jphotobiol.2016.12.008
Type of Journal (Indian or Foreign)Foreign
Impact Factor (IF)3.035
Divison category: 
Center for Material Characterization (CMC)

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