Determination of triazines and triazoles in grapes using atmospheric pressure matrix-assisted laser desorption/ionization high-resolution mass spectrometry
| Title | Determination of triazines and triazoles in grapes using atmospheric pressure matrix-assisted laser desorption/ionization high-resolution mass spectrometry |
| Publication Type | Journal Article |
| Year of Publication | 2017 |
| Authors | Mahale, V, Singh, A, Phadke, GS, Ghanate, AD, Oulkar, DP, Banerjee, K, Panchagnula, V |
| Journal | Journal of Aoac International |
| Volume | 100 |
| Issue | 3 |
| Pagination | 640-646 |
| Date Published | MAY |
| Type of Article | Article |
| Abstract | A chromatography-free atmospheric pressure matrix-assisted laser desorption/ionization high-resolution mass spectrometry (AP-MALDI HRMS) method is described for the simultaneous and quantitative detection of triazines and triazoles in grapes. The analytes were detected reproducibly with high mass accuracy (mass error within 5 ppm) and further confirmed by collision-induced dissociation fragmentation in tandem MS. The LODs and LOQs for all the analytes were found to be in the nanogram per gram level (15-20 ng/g LOQ). Internal standard normalized high-resolution accurate mass extracted (HR-AM) peak intensities of the detected ions were used to generate the concentration response curves. Linearity (with R-2 values around 0.99) was obtained for these curves within a concentration range of 20-200 ng/g of the individual analytes. The accuracy and precision of the method were further established using QC samples. Validation and performance comparison of the AP-MALDI HRMS method with an existing standard method using LC with :triple quadrupole MS was carried out (evaluating sensitivity, accuracy, precision, and analysis time) using 20 table-grape field samples after QuEChERS extraction. |
| DOI | 10.5740/jaoacint.17-0047 |
| Type of Journal (Indian or Foreign) | Foreign |
| Impact Factor (IF) | 0.918 |
Divison category:
Chemical Engineering & Process Development