Determination of triazines and triazoles in grapes using atmospheric pressure matrix-assisted laser desorption/ionization high-resolution mass spectrometry

TitleDetermination of triazines and triazoles in grapes using atmospheric pressure matrix-assisted laser desorption/ionization high-resolution mass spectrometry
Publication TypeJournal Article
Year of Publication2017
AuthorsMahale, V, Singh, A, Phadke, GS, Ghanate, AD, Oulkar, DP, Banerjee, K, Panchagnula, V
JournalJournal of Aoac International
Volume100
Issue3
Pagination640-646
Date PublishedMAY
Type of ArticleArticle
AbstractA chromatography-free atmospheric pressure matrix-assisted laser desorption/ionization high-resolution mass spectrometry (AP-MALDI HRMS) method is described for the simultaneous and quantitative detection of triazines and triazoles in grapes. The analytes were detected reproducibly with high mass accuracy (mass error within 5 ppm) and further confirmed by collision-induced dissociation fragmentation in tandem MS. The LODs and LOQs for all the analytes were found to be in the nanogram per gram level (15-20 ng/g LOQ). Internal standard normalized high-resolution accurate mass extracted (HR-AM) peak intensities of the detected ions were used to generate the concentration response curves. Linearity (with R-2 values around 0.99) was obtained for these curves within a concentration range of 20-200 ng/g of the individual analytes. The accuracy and precision of the method were further established using QC samples. Validation and performance comparison of the AP-MALDI HRMS method with an existing standard method using LC with :triple quadrupole MS was carried out (evaluating sensitivity, accuracy, precision, and analysis time) using 20 table-grape field samples after QuEChERS extraction.
DOI10.5740/jaoacint.17-0047
Type of Journal (Indian or Foreign)Foreign
Impact Factor (IF)0.918
Divison category: 
Chemical Engineering & Process Development

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