Determination of organic acid impurities in lactic acid obtained by fermentation of sugarcane juice

TitleDetermination of organic acid impurities in lactic acid obtained by fermentation of sugarcane juice
Publication TypeJournal Article
Year of Publication2011
AuthorsQureshi, MShadbar, Bhongale, SSitaram, Thorave, AK
JournalJournal of Chromatography A
Volume1218
Issue40
Pagination7147-7157
Date PublishedOCT
ISSN0021-9673
KeywordsLactic acid, Method validation, Organic acid impurities, Polar embedded RP-HPLC, Sugarcane juice fermentation
Abstract

Lactic acid produced by fermentation process mostly contains a number of aliphatic carboxylic acids as impurities. In this work, carboxylic acid impurities in lactic acid samples from a number of sources were determined at ppm levels. A simple HPLC method was developed that utilized a new generation polar embedded reverse phase, 20 mM phosphate buffer at pH 2.20 (+/- 0.05) and UV detection at 210 nm. The method enabled quantitative analysis of the above acids in lactic acid matrix. The experimental conditions for column temperature, mobile phase pH and flow rate were optimized. A detailed validation of the method was performed for linearity, precision, accuracy, selectivity, limit of detection (LOD), limit of quantitation (LOQ), ruggedness and repeatability and reproducibility (R&R). (C) 2011 Elsevier B.V. All rights reserved.

DOI10.1016/j.chroma.2011.08.025
Type of Journal (Indian or Foreign)Foreign
Impact Factor (IF)4.71
Divison category: 
Chemical Engineering & Process Development