Deinococcus enclensis sp nov., isolated from a marine sediment sample

TitleDeinococcus enclensis sp nov., isolated from a marine sediment sample
Publication TypeJournal Article
Year of Publication2015
AuthorsThorat, MN, Mawlankar, RR, Sonalkar, VV, V. Ramana, V, Joseph, N, Shouche, YS, Dastager, SGulam
JournalAntonie Van Leeuwenhoek International Journal of General and Molecular Microbiology
Volume107
Issue1
Pagination141-148
Date PublishedJAN
ISSN0003-6072
Keywords16S rRNA, Deinococcus, Marine sediments, Polyphasic
Abstract

A novel pale-pink coloured strain, designated NIO-1023(T), was isolated from a marine sediment sample from Chorao Island, Goa, India. The taxonomic position of strain NIO-1023(T) was investigated by using a polyphasic approach. The cells were observed to be Gram-stain positive, coccal shaped and non-spore forming. Phylogenetic analyses using the 16S rRNA gene sequence of the isolate indicated that the organism belongs to the genus Deinococcus. The strain NIO-1023(T) showed highest 16S rRNA gene sequence similarities with Deinococcus ficus (97.8 %), whereas other Deinococcus species showed less than 95 % sequence similarity. The DNA-DNA relatedness with respect to D. ficus CC-FR2-10(T) was 23.9 %. Chemotaxonomic data revealed that strain NIO-1023(T) contains only menaquinone MK-8 as the respiratory quinone and a complex polar lipid profile consisting of different unidentified glycolipids and polar lipids, two unknown phospholipids and three unknown phosphoglycolipids. As in other deinococci, one of these phosphoglycolipids was predominant in the profile. The predominant fatty acids were identified as C-17:1 w8c, C-16:1 w6c/w7c, C-15:1 w6c and C-17:1 w9c. The genomic DNA G + C content of strain NIO-1023(T) was determined to be 67.2 mol%. The biochemical and chemotaxonomic properties demonstrate that strain NIO-1023(T) represents a novel species, for which the name Deinococcus enclensis sp. nov. is proposed. The type strain is NIO-1023(T) (=DSM 25127(T) = NCIM 5456(T)).

DOI10.1007/s10482-014-0311-4
Type of Journal (Indian or Foreign)

Foreign

Impact Factor (IF)1.944
Divison category: 
National Collection of Industrial Micr-organisms (NCIM)