Deciphering the sensing of alpha-amyrin acetate with hs-DNA: a multipronged biological probe
| Title | Deciphering the sensing of alpha-amyrin acetate with hs-DNA: a multipronged biological probe |
| Publication Type | Journal Article |
| Year of Publication | 2022 |
| Authors | V. Pansare, A, Shedge, AA, Sonawale, MC, V. Pansare, S, Mahakal, AD, Khairkar, SR, Chhatre, SY, Kulal, DK, Patil, VR |
| Journal | RSC Advances |
| Volume | 12 |
| Issue | 3 |
| Pagination | 1238-1243 |
| Date Published | JAN |
| Type of Article | Article |
| Abstract | In this study, we focus on the biomimetic development of small molecules and their biological sensing with DNA. The binding of herring sperm deoxyribonucleic acid (hs-DNA) with naturally occurring bioactive small molecule alpha-amyrin acetate (alpha-AA), a biomimetic - isolated from the leaves of Ficus (F.) arnottiana is investigated. Collective information from various imaging, spectroscopic and biophysical experiments provides evidence that alpha-AA is a minor groove sensor of hs-DNA and preferentially binds to the A-T-rich regions. Interactions of different concentrations of small molecule alpha-AA with hsDNA were evaluated via various analytical techniques such as UV-Vis, circular dichroism (CD) and fluorescence emission spectroscopy. Fluorescence emission spectroscopy results suggest that alpha-AA decreases the emission level of hsDNA. DNA minor groove sensor Hoechst 33258 and intercalative sensor EB, melting transition analysis (T-M) and viscosity analysis clarified that alpha-AA binds to hs-DNA via a groove site. Biophysical chemistry and molecular docking studies show that hydrophobic interactions play a major role in this binding. The present research deals with a natural product biosynthesis-linked chemical-biology interface sensor as a biological probe for alpha-AA: hs-DNA. |
| DOI | 10.1039/d1ra07195e |
| Type of Journal (Indian or Foreign) | Foreign |
| Impact Factor (IF) | 3.361 |
Divison category:
Polymer Science & Engineering
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