De novo transcriptome analysis of the egg parasitoid Trichogramma chilonis Ishii (Hymenoptera: Trichogrammatidae): a biological control agent

TitleDe novo transcriptome analysis of the egg parasitoid Trichogramma chilonis Ishii (Hymenoptera: Trichogrammatidae): a biological control agent
Publication TypeJournal Article
Year of Publication2018
AuthorsKerima, OZ, Niranjana, P, Kumar, BSVinay, Ramachandrappa, R, Puttappa, S, Lalitha, Y, Jalali, SK, Ballal, CR, Thulasiram, HV
JournalGene Reports
Volume13
Pagination115-129
Date PublishedDEC
Type of ArticleArticle
ISSN24520144
AbstractTrichogramma chilonis Ishii is an endoparasitic wasp widely used as a biological control agent of economically important pests. However, absence of genetic information on T. chilonis hampers further research, especially at the molecular level. Here, we performed the first RNA-Seq of the adult T. chilonis. A total of 18,372,639 high-quality reads were generated using Illumina pair-ended sequencing platform. De novo assembly resulted into 24,488 transcripts, with mean length and N50 values of 1444.2 bp and 2667 bp, respectively. Of the transcripts, 14,643 exhibited putative homology with class Insecta proteins from UniPROT database. To understand the contig functions, gene descriptions, gene ontology and cluster of orthologous group annotations were analyzed. Following functional annotation, a number of transcripts encoding detoxification, target receptor action and olfaction- related proteins were identified and analyzed. In addition, 4520 transcripts containing simple sequence repeats, ranging from dinucleotide to hexanucleotide repeats were discovered, with trinucleotide being the most enriched repeats. This new sequence resource will shed light on various molecular mechanisms in T. chilonis and related species.
DOI10.1016/j.genrep.2018.08.009
Type of Journal (Indian or Foreign)Foreign
Impact Factor (IF)0.216
Divison category: 
Organic Chemistry

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