D-(-)-Lactic acid production from cellobiose and cellulose by Lactobacillus lactis mutant RM2-24

TitleD-(-)-Lactic acid production from cellobiose and cellulose by Lactobacillus lactis mutant RM2-24
Publication TypeJournal Article
Year of Publication2010
AuthorsSinghvi, M, Joshi, D, Adsul, MG, Varma, A, Gokhale, DV
JournalGreen Chemistry
Volume12
Issue6
Pagination1106-1109
Date PublishedMAY
ISSN1463-9262
Abstract

Lactobacillus lactis mutant RM2-24 utilizes cellobiose efficiently, converting it into D-(-)-lactic acid. Cellobiose-degrading enzyme activities were determined for whole cells, cell extracts and disrupted cells. Aryl-beta-glucosidase activity was detected in whole cells and disrupted cells, suggesting that these activities are confined to the cells. The mutant produced 80 g l(-1) of lactic acid from 100 g l(-1) of cellobiose with 1.66 g l(-1) h(-1) productivity. Production of D-lactic acid from different cellulose samples was also studied. The cellulose samples at high concentration (10%) were hydrolyzed by cellulase enzyme preparation (10 FPU g(-1) cellulose) derived from Penicillium janthinellum mutant EU1 generated in our own laboratory. We obtained a maximum 72% hydrolysis, yielding glucose and cellobiose as the main end products. Lactic acid was produced from these cellulose samples by simultaneous sacchari. cation and fermentation (SSF) in a media containing a cellulase enzyme preparation derived from Penicillium janthinellum mutant EU1 and cellobiose utilizing Lactobacillus lactis mutant RM2-24. A maximum lactic acid concentration of 73 g l(-1) was produced from a concentration of 100 g l(-1) of bagasse-derived cellulose, the highest productivity and yield being 1.52 g l(-1) h(-1) and 0.73 g g(-1), respectively. Considering that bagasse is a waste material available in abundance, we propose to use this biomass to produce cellulose and then sugars, which can be fermented to valuable products such as ethanol and lactic acid.

DOI10.1039/b925975a
Type of Journal (Indian or Foreign)

Foreign

Impact Factor (IF)

5.472

Divison category: 
National Collection of Industrial Micr-organisms (NCIM)