Culture conditions for the production of thermostable lipase by thermomyces lanuginosus

TitleCulture conditions for the production of thermostable lipase by thermomyces lanuginosus
Publication TypeJournal Article
Year of Publication2016
AuthorsSreelatha, B, Vankudoth, KRao, R. Kumar, R, Girisham, S, Reddy, SM
JournalJournal of Basic and Applied Sciences
Date PublishedNOV
Abstract

In the present investigation lipase production by three strains of thermophilic Thermomyces lanuginosus (GSLMBKU-10, GSLMBKU-13 and GSLMBKU-14) was carried out in submerged fermentation process. Olive oil and triacetin (0.1%) were added to the basal medium, which stimulated the lipase production. The maximum lipase was produced by GSLMBKU-10 and GSLMBKU-13 in yeast extract starch medium supplemented with triacetin (0.1%). The optimum pH was recorded at 6.0, 6.5 and 7.0 by GSLMBKU-10, GSLMBKU-13 and GSLMBKU-14 respectively. T. lanuginosus GSLMBKU-10 strains failed to produce lipase at pH 8.0. The optimum temperature for lipase production was observed at 45 °C by GSLMBKU-14 and GSLMBKU-10, while that for GSLMBKU-13 was at 50 °C. The marginal temperature ranged from 45 °C to 50 °C for both lipase production and vegetative growth by the three strains of T. lanuginosus under study. In conclusion, the GSLMBKU-13 strain was comparatively superior in the production of lipase than the other two strains under investigation.

DOI10.1016/j.bjbas.2016.11.010
Type of Journal (Indian or Foreign)

Foreign

Divison category: 
Biochemical Sciences