Conversion of dried aspergillus candidus mycelia grown on waste whey to biodiesel by in situ acid transesterification

TitleConversion of dried aspergillus candidus mycelia grown on waste whey to biodiesel by in situ acid transesterification
Publication TypeJournal Article
Year of Publication2015
AuthorsKakkad, H, Khot, M, Zinjarde, SS, RaviKumar, A, V. Kumar, R, Kulkarni, BD
JournalBioresource Technology
Volume197
Pagination502-507
Date PublishedDEC
ISSN0960-8524
KeywordsAspergillus candidus, Biodiesel, In situ acid transesterification, Plackett-Burman Design, Whey
Abstract

This study reports optimization of the transesterification reaction step on dried biomass of an oleaginous fungus Aspergillus candidus grown on agro-dairy waste, whey. Acid catalyzed transesterification was performed and variables affecting esterification, viz., catalyst methanol and chloroform concentrations, temperature, time, and biomass were investigated. Statistical optimization of the transesterification reaction using Plackett-Burman Design showed biomass to be the predominant factor with a 12.5-fold increase in total FAME from 25.6 to 320 mg. Studies indicate that the transesterification efficiency in terms of conversion is favored by employing lower biomass loadings. A. candidus exhibited FAME profiles containing desirable saturated (30.2%), monounsaturated (31.5%) and polyunsaturated methyl esters (38.3%). The predicted and experimentally determined biodiesel properties (density, kinematic viscosity, iodine value, cetane number, TAN, water content, total and free glycerol) were in accordance with international (ASTM D6751, EN 14214) and national (IS 15607) standards. (C) 2015 Published by Elsevier Ltd.

DOI10.1016/j.biortech.2015.07.118
Type of Journal (Indian or Foreign)

Foreign

Impact Factor (IF)4.917
Divison category: 
Chemical Engineering & Process Development