Complex carbohydrate specificity of lectin from fruiting body of ganoderma lucidum. a surface plasmon resonance study

TitleComplex carbohydrate specificity of lectin from fruiting body of ganoderma lucidum. a surface plasmon resonance study
Publication TypeJournal Article
Year of Publication2007
AuthorsThakur, A, Pal, L, Ahmad, A, Khan, MIslam
JournalIUBMB Life
Volume59
Issue12
Pagination758-764
Date PublishedJAN
Type of ArticleArticle
ISSN1521-6543
Keywordsganoderma lucidum, glycans, Lectin, SPR
Abstract

The thermodynamics and kinetics of binding of glycans and glycoproteins to Ganoderma lucidum lectin was studied using surface plasmon resonance. The lectin showed highest affinity for asialo triantennary N glycan (Ka = 3.52 x 10(5)) among the glycans tested. There was a several fold increase in affinity for glycoproteins compared to their corresponding glycans and coincident increase in contribution from enthalpy (Delta H), suggesting the involvement of hydrogen bonding in the interaction as well as involvement of protein-protein interactions. Increased affinity also showed increase in unfavorable negative binding entropy (Delta S) which was compensated with higher enthalpy. The glycoproteins showed faster association rates (k(1)) and the activation energy (E-1(double dagger)) in the association process was much lower for the glycoproteins than glycans, resulting in their faster associations. These observations elaborate the role of protein matrix in lectin-glycoconjugate interaction.

DOI10.1080/15216540701663463
Type of Journal (Indian or Foreign)

Foreign

Impact Factor (IF)

2.653

Divison category: 
Biochemical Sciences