Cell line development for biomanufacturing processes: recent advances and an outlook

TitleCell line development for biomanufacturing processes: recent advances and an outlook
Publication TypeJournal Article
Year of Publication2015
AuthorsLe, H, Vishwanathan, N, Jacob, NM, Gadgil, M, Hu, W-S
JournalBiotechnology Letters
Volume37
Issue8
Pagination1553-1564
Date PublishedAUG
ISSN0141-5492
KeywordsBiomanufacturing, Biosimilars, Cell line development, CHO cells genomics, Genome editing, Transcriptomics
Abstract

At the core of a biomanufacturing process for recombinant proteins is the production cell line. It influences the productivity and product quality. Its characteristics also dictate process development, as the process is optimized to complement the producing cell to achieve the target productivity and quality. Advances in the past decade, from vector design to cell line screening, have greatly expanded our capability to attain producing cell lines with certain desired traits. Increasing availability of genomic and transcriptomic resources for industrially important cell lines coupled with advances in genome editing technology have opened new avenues for cell line development. These developments are poised to help biosimilar manufacturing, which requires targeting pre-defined product quality attributes, e.g., glycoform, to match the innovator's range. This review summarizes recent advances and discusses future possibilities in this area.

DOI10.1007/s10529-015-1843-z
Type of Journal (Indian or Foreign)

Foreign

Impact Factor (IF)1.639
Divison category: 
Chemical Engineering & Process Development