Bioactive thermoresponsive polyblend nanofiber formulations for wound healing

TitleBioactive thermoresponsive polyblend nanofiber formulations for wound healing
Publication TypeJournal Article
Year of Publication2015
AuthorsPawar, MD, Rathna, GVN, Agrawal, S, Kuchekar, BS
JournalMaterials Science & Engineering C-Materials for Biological Applications
Volume48
Pagination126-137
Date PublishedMAR
Type of ArticleArticle
ISSN0928-4931
KeywordsAntibacterial, Cell viability, drug release, nanofibers, thermoresponsive, wound Healing
Abstract

The rationale of this work is to develop new bioactive thermoresponsive polyblend nanofiber formulations for wound healing (topical). Various polymer compositions of thermoresponsive, poly(N-isopropylacrylamide), egg albumen and poly(epsilon-caprolactone) blend solutions with and without a drug [gatifloxacin hydrochloride, Gati] were prepared. Non-woven nanofibers of various compositions were fabricated using an electrospinning technique. The morphology of the nanofibers was analyzed by an environmental scanning electron microscope. The morphology was influenced by the concentration of polymer, drug, and polymer blend composition. Fourier transform infrared spectroscopy analysis showed the shift in bands due to hydrogen ion interactions between polymers and drug. Thermogram of PNIPAM/PCL/EA with Gati recorded a shift in lower critical solution temperature (LCST) and glass transition temperature (T-g) of PNIPAM. Similarly T-g and melting temperature (T-m) of PCL were shifted. X-ray diffraction patterns recorded a decrease in the crystalline state of PCL nanofibers and transformed crystalline drug to an amorphous state. In vitro release study of nanofibers with Gati showed initial rapid release up to 10 h, followed by slow and controlled release for 696 h (29 days). Nanofiber mats with Gati exhibited antibacterial properties to Staphylococcus aureus, supported suitable controlled drug release with in vitro cell viability and in vivo wound healing. (C) 2014 Elsevier B.V. All rights reserved.

DOI10.1016/j.msec.2014.11.037
Type of Journal (Indian or Foreign)Foreign
Impact Factor (IF)4.628
Divison category: 
Polymer Science & Engineering

Add new comment