<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Yadav, Priya</style></author><author><style face="normal" font="default" size="100%">Shahane, Ganesh</style></author><author><style face="normal" font="default" size="100%">Ramasamy, Sureshkumar</style></author><author><style face="normal" font="default" size="100%">Sengupta, Durba</style></author><author><style face="normal" font="default" size="100%">Gaikwad, Sushama</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Structural-functional insights and studies on saccharide binding of Sophora japonica seed lectin</style></title><secondary-title><style face="normal" font="default" size="100%">International Journal of Biological Macromolecules</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Dissociation</style></keyword><keyword><style  face="normal" font="default" size="100%">docking</style></keyword><keyword><style  face="normal" font="default" size="100%">Homology model</style></keyword><keyword><style  face="normal" font="default" size="100%">Ligand binding</style></keyword><keyword><style  face="normal" font="default" size="100%">Sophora japonica seed lectin</style></keyword><keyword><style  face="normal" font="default" size="100%">Thermal transitions</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2016</style></year><pub-dates><date><style  face="normal" font="default" size="100%">OCT</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">91</style></volume><pages><style face="normal" font="default" size="100%">75-84</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Functional and conformational transitions of the Sophora japonica seed lectin (SJL) were studied in detail using bioinformatics and biophysical tools. Homology model of the lectin displayed all the characteristics of the legume lectin monomer and the experimental observations correlated well with the structural information. In silico studies were performed by protein-ligand docking, calculating the respective binding energies and the residues involved in the interactions were derived from LigPlot(+) analysis. Fluorescence titrations showed three times higher affinity of T-antigen disaccharide than N-acetyl galactosamine (GaINAc) towards SJL indicating extended sugar binding site of the lectin. Thermodynamic parameters of T-antigen binding to SJL indicated the process to be endothermic and entropically driven while those of GaINAc showed biphasic process. SDS-PAGE showed post-translationally modified homotetrameric species of the lectin under native conditions. In presence of guanidine hydrochloride (0.5-5.0 M), the tetramer first dissociated into dimers followed by unfolding of the protein as indicated by size exclusion chromatography, fluorescence and CD spectroscopy. Different structural rearrangements were observed during thermal denaturation of SJL at physiological pH 7.2, native pH 8.5 and molten globule inducing pH 1.0. Topological information revealed by solute quenching studies at respective pH indicated differential hydrophobic environment and charge density around tryptophan residues. (C) 2016 Elsevier B.V. All rights reserved.&lt;/p&gt;</style></abstract><work-type><style face="normal" font="default" size="100%">Article</style></work-type><custom3><style face="normal" font="default" size="100%">Foreign</style></custom3><custom4><style face="normal" font="default" size="100%">3.138</style></custom4></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Gaurav Bhattacharjee</style></author><author><style face="normal" font="default" size="100%">Vivek Barmecha</style></author><author><style face="normal" font="default" size="100%">Nilesh Choudhary</style></author><author><style face="normal" font="default" size="100%">Nawal K. Pande</style></author><author><style face="normal" font="default" size="100%">Parivesh Chugh</style></author><author><style face="normal" font="default" size="100%">Rajnish Kumar</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Methane hydrate dissociation in the presence of novel benign additives</style></title><secondary-title><style face="normal" font="default" size="100%">Energy Procedia</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Depressurization</style></keyword><keyword><style  face="normal" font="default" size="100%">Dissociation</style></keyword><keyword><style  face="normal" font="default" size="100%">Energy Ratio</style></keyword><keyword><style  face="normal" font="default" size="100%">kinetics</style></keyword><keyword><style  face="normal" font="default" size="100%">LHDP</style></keyword><keyword><style  face="normal" font="default" size="100%">methane hydrate</style></keyword><keyword><style  face="normal" font="default" size="100%">Thermal Efficiency</style></keyword><keyword><style  face="normal" font="default" size="100%">thermal stimulation</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2019</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.sciencedirect.com/science/article/pii/S1876610219305648</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">158</style></volume><pages><style face="normal" font="default" size="100%">5856 - 5865</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;The apparent drawbacks of the classical approaches towards dissociation of natural gas hydrates have resulted in a paradigm shift into the development of new hybrid hydrate dissociation practices combining the various basic hydrate dissociation techniques. Another approach that can be followed to maximize the efficiency of gas production from natural gas hydrate reserves is the identification of benign additives which when used even in sparingly small concentrations may enhance the kinetics of hydrate dissociation. In the present work, a class of such additives, never reported before, have been unveiled and christened as Low Dosage Hydrate Dissociation Promoters (LHDPs). The additives were first short listed from a wide potential pool using a lab scale ( 250 ml) stirred tank reactor setup and then further studied using a bench scale ( 2.35 l) reactor setup where they were injected in the form of a water-additive stream to dissociate hydrates. The dissociation approach followed in the case of the bench scale reactor experiments was a combination of the thermal stimulation and depressurization processes along with the element of injection of additives. For both sets of experiments (lab and bench scale), the newly identified LHDPs were found to enhance the kinetics of methane hydrate dissociation as compared to pure water. It was observed that concentration of additive and its flow rate also affect the kinetics of methane hydrate dissociation. An energy and efficiency analysis for the hydrate dissociation method in the case of bench scale rector revealed that additive presence enhanced the energy ratio and thermal efficiency four fold as compared to pure water.&lt;/p&gt;
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