<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Nupura, Hirve</style></author><author><style face="normal" font="default" size="100%">Asmita, Tingare</style></author><author><style face="normal" font="default" size="100%">Sharat, B.</style></author><author><style face="normal" font="default" size="100%">Asmita, Prabhune</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Media optimization for the production of cephalosporin C acylase from a novel bacterial source: alcaligenes xylosoxidans MTCC*491</style></title><secondary-title><style face="normal" font="default" size="100%">Research Journal of Biotechnology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">alcaligenes xylosoxidans MTCC*491</style></keyword><keyword><style  face="normal" font="default" size="100%">cephalosporin C (CPC)</style></keyword><keyword><style  face="normal" font="default" size="100%">cephalosporin C acylase (CCA)</style></keyword><keyword><style  face="normal" font="default" size="100%">fermentation carbon source</style></keyword><keyword><style  face="normal" font="default" size="100%">nitrogen source</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2008</style></year><pub-dates><date><style  face="normal" font="default" size="100%">FEB</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">1</style></number><publisher><style face="normal" font="default" size="100%">RESEARCH JOURNAL BIOTECHNOLOGY</style></publisher><pub-location><style face="normal" font="default" size="100%">SECTOR A-80, SCHEME NO 54, VIJAY NAGAR, A B ROAD, INDORE, 452 010 MP, INDIA</style></pub-location><volume><style face="normal" font="default" size="100%">3</style></volume><pages><style face="normal" font="default" size="100%">16-21</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;The Gram-negative bacterium Alcaligenes xylosoxidans MTCC *491 has been studied for the production of cephalosporin C acylase (CCA), a pharmaceutically important enzyme. The enzyme hydrolyses cephalosporin C (CPC), a natural product, to 7-aminocephalosporanic acid, a key intermediate in the production of semisynthetic cephalosporin antibiotics. Effect of medium, pH, temperature, carbon source, nitrogen source and incubation time, on the production of CCA was studied. 7.7 IU/ml activity was obtained when Luria Bertani broth (LB) was used as a fermentation medium. Glucose and galactose increased the enzyme activity by 9 % and 22 %, while all the nitrogen sources tested showed a repressive effect. Maximum CCA activity was observed around the 5(th) day of incubation indicating potential role of CCA in secondary metabolism. pH 8.0 and 40 degrees C were found to be the optimum conditions for acylase activity.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><custom3><style face="normal" font="default" size="100%">Foreign</style></custom3><custom4><style face="normal" font="default" size="100%">0.08</style></custom4></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Alejandra Pompa-Monroy, Daniella</style></author><author><style face="normal" font="default" size="100%">Leticia Iglesias, Ana</style></author><author><style face="normal" font="default" size="100%">Gulam Dastager, Syed</style></author><author><style face="normal" font="default" size="100%">Namdeo Thorat, Meghana</style></author><author><style face="normal" font="default" size="100%">Olivas-Sarabia, Amelia</style></author><author><style face="normal" font="default" size="100%">Valdez-Castro, Ricardo</style></author><author><style face="normal" font="default" size="100%">Angelica Hurtado-Ayala, Lilia</style></author><author><style face="normal" font="default" size="100%">Manuel Cornejo-Bravo, Jose</style></author><author><style face="normal" font="default" size="100%">Lizeth Perez-Gonzalez, Graciela</style></author><author><style face="normal" font="default" size="100%">Jesus Villarreal-Gomez, Luis</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Comparative study of polycaprolactone electrospun fibers and casting films enriched with carbon and nitrogen sources and their potential use in water bioremediation</style></title><secondary-title><style face="normal" font="default" size="100%">Membranes</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">bacterial growth</style></keyword><keyword><style  face="normal" font="default" size="100%">carbon source</style></keyword><keyword><style  face="normal" font="default" size="100%">electrospinning</style></keyword><keyword><style  face="normal" font="default" size="100%">nitrogen source</style></keyword><keyword><style  face="normal" font="default" size="100%">poly (caprolactone)</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2022</style></year><pub-dates><date><style  face="normal" font="default" size="100%">MAR</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">327</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;
	Augmenting bacterial growth is of great interest to the biotechnological industry. Hence, the effect of poly (caprolactone) fibrous scaffolds to promote the growth of different bacterial strains of biological and industrial interest was evaluated. Furthermore, different types of carbon (glucose, fructose, lactose and galactose) and nitrogen sources (yeast extract, glycine, peptone and urea) were added to the scaffold to determinate their influence in bacterial growth. Bacterial growth was observed by scanning electron microscopy; thermal characteristics were also evaluated; bacterial cell growth was measured by ultraviolet-visible spectrophotometry at 600-nm. Fibers produced have an average diameter between 313 to 766 nm, with 44% superficial porosity of the scaffolds, a glass transition around similar to 64 degrees C and a critical temperature of similar to 338 degrees C. The fibrous scaffold increased the cell growth of Escherichia coli by 23% at 72 h, while Pseudomonas aeruginosa and Staphylococcus aureus increased by 36% and 95% respectively at 48 h, when compared to the normal growth of their respective bacterial cultures. However, no significant difference in bacterial growth between the scaffolds and the casted films could be observed. Cell growth depended on a combination of several factors: type of bacteria, carbon or nitrogen sources, casted films or 3D scaffolds. Microscopy showed traces of a biofilm formation around 3 h in culture of P. aeruginosa. Water bioremediation studies showed that P. aeruginosa on poly (caprolactone)/Glucose fibers was effective in removing 87% of chromium in 8 h.&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><work-type><style face="normal" font="default" size="100%">Article</style></work-type><custom3><style face="normal" font="default" size="100%">&lt;p&gt;
	Foreign&lt;/p&gt;
</style></custom3><custom4><style face="normal" font="default" size="100%">&lt;p&gt;
	4.562&lt;/p&gt;
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