<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Adsul, Mukund G.</style></author><author><style face="normal" font="default" size="100%">Ghule, J. E.</style></author><author><style face="normal" font="default" size="100%">Shaikh, H.</style></author><author><style face="normal" font="default" size="100%">Singh, R.</style></author><author><style face="normal" font="default" size="100%">Bastawade, Kulbhushan B.</style></author><author><style face="normal" font="default" size="100%">Gokhale, D. V.</style></author><author><style face="normal" font="default" size="100%">Varma, Anjanikumar</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Enzymatic hydrolysis of delignified bagasse polysaccharides</style></title><secondary-title><style face="normal" font="default" size="100%">Carbohydrate Polymers</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">bagasse polysaccharides</style></keyword><keyword><style  face="normal" font="default" size="100%">Cellulase</style></keyword><keyword><style  face="normal" font="default" size="100%">chemical treatment</style></keyword><keyword><style  face="normal" font="default" size="100%">Enzymatic hydrolysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Penicillium janthinellum</style></keyword><keyword><style  face="normal" font="default" size="100%">Sugarcane bagasse</style></keyword><keyword><style  face="normal" font="default" size="100%">Xylanase</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2005</style></year><pub-dates><date><style  face="normal" font="default" size="100%">OCT</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">1</style></number><publisher><style face="normal" font="default" size="100%">ELSEVIER SCI LTD</style></publisher><pub-location><style face="normal" font="default" size="100%">THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND</style></pub-location><volume><style face="normal" font="default" size="100%">62</style></volume><pages><style face="normal" font="default" size="100%">6-10</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Sugarcane bagasse, consisting of cellulose, xylan, and lignin, was chemically treated to generate bagasse samples with continuously decreasing content of lignin. These bagasse samples were hydrolyzed by cellulase and xylanase enzymes, produced earlier by Penicillium janthinellum NCIM 1171 in the same bagasse polysaccharides production medium. The hydrolysis was carried out by using different concentrations of the enzymes at two different temperatures, 30 and 50 degrees C, taking hydrolysis of Avicel as control. It was found that while the maximum hydrolysis for Avicel was 70% that of some of the bagasse polysaccharides was as high as 95%. The products of hydrolysis were glucose, xylose, and arabinose, as confirmed by high pressure ion chromatography (HPIC). It is interesting to note that arabinose, which constitutes about 10% of the weight of bagasse xylan, could also be released easily by the enzymes. Also, the initial rates of hydrolysis was found to be much higher for the bagasse polysaccharides, and in some cases about 90% of the hydrolysis occurred within 20 h. Amongst all bagasse samples, the sample with (Kappa no. 1.2, lignin content 0.18%) gave the highest degree of hydrolysis at 50 degrees C. Even the bagasse polysaccharide with Kappa no. 16.8 (lignin content 2.5%) underwent greater extent of hydrolysis than Avicel. Apparently, the delignified bagasse medium appears to be a facile medium for the combined hydrolytic action of the cellulase and xylanase enzymes. Considering that sugarcane bagasse is a waste biomass material available in abundance annually, this methodology can be used to value-add to this biomass to produce sugars, which can be fermented to produce biofuels like ethanol. (C) 2005 Elsevier Ltd. All rights reserved.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><work-type><style face="normal" font="default" size="100%">Article</style></work-type><custom3><style face="normal" font="default" size="100%">&lt;p&gt;Foreign&lt;/p&gt;</style></custom3><custom4><style face="normal" font="default" size="100%">&lt;p&gt;4.219&lt;/p&gt;</style></custom4></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Adsul, Mukund G.</style></author><author><style face="normal" font="default" size="100%">Bastawade, Kulbhushan B.</style></author><author><style face="normal" font="default" size="100%">Varma, Anjanikumar</style></author><author><style face="normal" font="default" size="100%">Gokhale, D. V.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Strain improvement of penicillium janthinellum ncim 1171 for increased cellulase production</style></title><secondary-title><style face="normal" font="default" size="100%">Bioresource Technology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">2-deoxy-D-glucose resistant mutants</style></keyword><keyword><style  face="normal" font="default" size="100%">cellulase activity</style></keyword><keyword><style  face="normal" font="default" size="100%">EMS and ultraviolet mutation</style></keyword><keyword><style  face="normal" font="default" size="100%">Penicillium janthinellum</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2007</style></year><pub-dates><date><style  face="normal" font="default" size="100%">MAY</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">7</style></number><publisher><style face="normal" font="default" size="100%">ELSEVIER SCI LTD</style></publisher><pub-location><style face="normal" font="default" size="100%">THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND</style></pub-location><volume><style face="normal" font="default" size="100%">98</style></volume><pages><style face="normal" font="default" size="100%">1467-1473</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;The strain of Penicilhuinjanthinellunt NOM 1171 was subjected to mutation involving treatment of Ethyl Methyl Sulfonate (EMS) for 24 h followed by UV-irradiation for 3 min. Successive mutants showed enhanced cellulase production (EMS-UV-8), clearance zone on Avicel containing plate (SM2) and rapid growth on Walseth cellulose agar plates containing 0.2% 2-deoxy-D-glucose (SM3). These mutants were transferred to Walseth cellulose plates containing higher concentration (1.5%) of 2-deoxy-D-glucose (SM4) in which only five mutants showed clearance zone on SM4. All these mutants showed approximately two-fold increase in activity of both FPase and CMCase in shake flask culture when grown on basal medium containing CP-123 (1%) and wheat bran (2.5%). The enzyme preparations from these mutants were used to hydrolyze Avicel. Higher hydrolysis yields of Avicel were obtained with enzyme preparations of EU1. This is the first report on the isolation and selection of mutants based on hydrolysis of Avicel, which is the most crystalline substrate. (c) 2006 Elsevier Ltd. All rights reserved.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">7</style></issue><work-type><style face="normal" font="default" size="100%">Article</style></work-type><custom3><style face="normal" font="default" size="100%">&lt;p&gt;Foreign&lt;/p&gt;</style></custom3><custom4><style face="normal" font="default" size="100%">&lt;p&gt;4.917&lt;/p&gt;</style></custom4></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Rao, Mala</style></author><author><style face="normal" font="default" size="100%">Varma, Anjanikumar</style></author><author><style face="normal" font="default" size="100%">Deshmukh, Sumedha S.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Production of single cell protein, essential amino acids, and xylanase by penicillium janthinellum</style></title><secondary-title><style face="normal" font="default" size="100%">Bioresources</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Bagasse hydrolysate</style></keyword><keyword><style  face="normal" font="default" size="100%">Crude protein</style></keyword><keyword><style  face="normal" font="default" size="100%">Microbial biomass production</style></keyword><keyword><style  face="normal" font="default" size="100%">Penicillium janthinellum</style></keyword><keyword><style  face="normal" font="default" size="100%">Xylanase</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2010</style></year><pub-dates><date><style  face="normal" font="default" size="100%">OCT</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">4</style></number><publisher><style face="normal" font="default" size="100%">NORTH CAROLINA STATE UNIV DEPT WOOD &amp; PAPER SCI</style></publisher><pub-location><style face="normal" font="default" size="100%">CAMPUS BOX 8005, RALEIGH, NC 27695-8005 USA</style></pub-location><volume><style face="normal" font="default" size="100%">5</style></volume><pages><style face="normal" font="default" size="100%">2470-2477</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Microbial biomass having 46% crude protein content and enriched with essential amino acids as well as extracellular xylanase activity (100-150 IU/ml) was produced by an efficient fungal strain, Penicillium janthinellum (NCIM St-F-3b). Optimization studies for maximum xylanase and biomass production showed that the fungus required a simple medium containing bagasse hemicellulose as carbon source and ammonium sulphate as the nitrogen source. Therefore bagasse, which is a waste product of the sugar industry, can be efficiently used in microbioal biomass protein preparation for animal feed.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">4</style></issue><custom3><style face="normal" font="default" size="100%">&lt;p&gt;Foreign&lt;/p&gt;</style></custom3><custom4><style face="normal" font="default" size="100%">&lt;p&gt;1.418&lt;/p&gt;</style></custom4></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sreeja-Raju, Athiraraj</style></author><author><style face="normal" font="default" size="100%">Christopher, Meera</style></author><author><style face="normal" font="default" size="100%">Kooloth-Valappil, Prajeesh</style></author><author><style face="normal" font="default" size="100%">Kuni-Parambil, Rajasree</style></author><author><style face="normal" font="default" size="100%">Gokhale, Digambar Vittal</style></author><author><style face="normal" font="default" size="100%">Sankar, Meena</style></author><author><style face="normal" font="default" size="100%">Abraham, Amith</style></author><author><style face="normal" font="default" size="100%">Pandey, Ashok</style></author><author><style face="normal" font="default" size="100%">Sukumaran, Rajeev K.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Penicillium janthinellum NCIM1366 shows improved biomass hydrolysis and a larger number of CAZymes with higher induction levels over Trichoderma reesei RUT-C30</style></title><secondary-title><style face="normal" font="default" size="100%">Biotechnology for Biofuels</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Bioethanol</style></keyword><keyword><style  face="normal" font="default" size="100%">CAZymes</style></keyword><keyword><style  face="normal" font="default" size="100%">Cellulase</style></keyword><keyword><style  face="normal" font="default" size="100%">Penicillium janthinellum</style></keyword><keyword><style  face="normal" font="default" size="100%">Secretome</style></keyword><keyword><style  face="normal" font="default" size="100%">Trichoderma reesei</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">DEC </style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">13</style></volume><pages><style face="normal" font="default" size="100%">196</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Background Major cost of bioethanol is attributed to enzymes employed in biomass hydrolysis. Biomass hydrolyzing enzymes are predominantly produced from the hyper-cellulolytic mutant filamentous fungus Trichoderma reesei RUT-C30. Several decades of research have failed to provide an industrial grade organism other than T. reesei, capable of producing higher titers of an effective synergistic biomass hydrolyzing enzyme cocktail. Penicillium janthinellum NCIM1366 was reported as a cellulase hyper producer and a potential alternative to T. reesei, but a comparison of their hydrolytic performance was seldom attempted. Results Hydrolysis of acid or alkali-pretreated rice straw using cellulase enzyme preparations from P. janthinellum and T. reesei indicated 37 and 43% higher glucose release, respectively, with P. janthinellum enzymes. A comparison of these fungi with respect to their secreted enzymes indicated that the crude enzyme preparation from P. janthinellum showed 28% higher overall cellulase activity. It also had an exceptional tenfold higher beta-glucosidase activity compared to that of T. reesei, leading to a lower cellobiose accumulation and thus alleviating the feedback inhibition. P. janthinellum secreted more number of proteins to the extracellular medium whose total concentration was 1.8-fold higher than T. reesei. Secretome analyses of the two fungi revealed higher number of CAZymes and a higher relative abundance of cellulases upon cellulose induction in the fungus. Conclusions The results revealed the ability of P. janthinellum for efficient biomass degradation through hyper cellulase production, and it outperformed the established industrial cellulase producer T. reesei in the hydrolysis experiments. A higher level of induction, larger number of secreted CAZymes and a high relative proportion of BGL to cellulases indicate the possible reasons for its performance advantage in biomass hydrolysis.&lt;/p&gt;
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