<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Rizvi, Masood Ahmad</style></author><author><style face="normal" font="default" size="100%">Dangat, Yuvraj B.</style></author><author><style face="normal" font="default" size="100%">Yaseen, Zahid</style></author><author><style face="normal" font="default" size="100%">Gupta, Vivek</style></author><author><style face="normal" font="default" size="100%">Khan, Khaliquz Zaman</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Synthesis, crystal structure and in vitro DNA binding studies of combretastatin A-4 analogue</style></title><secondary-title><style face="normal" font="default" size="100%">Croatica Chemica Acta</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">benzil</style></keyword><keyword><style  face="normal" font="default" size="100%">bio-physical</style></keyword><keyword><style  face="normal" font="default" size="100%">combretastatin A-4</style></keyword><keyword><style  face="normal" font="default" size="100%">crystal structure</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA binding</style></keyword><keyword><style  face="normal" font="default" size="100%">ethidium bromide</style></keyword><keyword><style  face="normal" font="default" size="100%">fluorescence</style></keyword><keyword><style  face="normal" font="default" size="100%">phenazones</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2015</style></year><pub-dates><date><style  face="normal" font="default" size="100%">DEC</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">3</style></number><publisher><style face="normal" font="default" size="100%">CROATIAN CHEMICAL SOC</style></publisher><pub-location><style face="normal" font="default" size="100%">MARULICEV TRG 19/II, 41001 ZAGREB, CROATIA</style></pub-location><volume><style face="normal" font="default" size="100%">88</style></volume><pages><style face="normal" font="default" size="100%">289-296</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Synthesis of a novel Combretastatin A-4 analogue using Schiff's reaction of benzil and 4-aminoantipyrine has been achieved under solvent free conditions. The structure of compound was examined spectroscopically and confirmed from single crystal diffraction studies. The synthesized Combretastatin A-4 analogue was investigated for its DNA binding ability as the plausible mechanism for its antitumor activity. The binding propensity of the synthesized compound with calf-thymus (CT) DNA was monitored with absorption and emission spectrophotometric titrations. The calculations predict a binding constant of 7.24 x 10(4) for the complex of the synthesized compound with CT DNA which is comparable in magnitude to that of DNA binding of bactericidal drug enoxacin and typical intercalation indicator ethidium bromide (EB). Competitive binding studies of the synthesized compound with EB using fluorescence titration reveal that it displaces the DNA-bound EB and binds in intercalative mode which was further supported by circular dichroism (CD) spectroscopy. The probable site and binding energy of the compound with DNA was further theoretically investigated by molecular docking studies. The significant DNA binding ability of the synthesized Combretastatin A4 analogue as revealed from this study could be related to the anticancer activity of the Combretastatin A4.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><custom3><style face="normal" font="default" size="100%">&lt;p&gt;Foreign&lt;/p&gt;</style></custom3><custom4><style face="normal" font="default" size="100%">0.732</style></custom4></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Usman, Mohammad</style></author><author><style face="normal" font="default" size="100%">Arjmand, Farukh</style></author><author><style face="normal" font="default" size="100%">Khan, Rais Ahmad</style></author><author><style face="normal" font="default" size="100%">Alsalme, Ali</style></author><author><style face="normal" font="default" size="100%">Ahmad, Musheer</style></author><author><style face="normal" font="default" size="100%">Sen Bishwas, Mousumi</style></author><author><style face="normal" font="default" size="100%">Tabassum, Sartaj</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Tetranuclear cubane Cu4O4 complexes as prospective anticancer agents: Design, synthesis, structural elucidation, magnetism, computational and cytotoxicity studies</style></title><secondary-title><style face="normal" font="default" size="100%">Inorganic Chimica Acta</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cu4O4 cubane complexes</style></keyword><keyword><style  face="normal" font="default" size="100%">Cytotoxicity</style></keyword><keyword><style  face="normal" font="default" size="100%">DFT</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA binding</style></keyword><keyword><style  face="normal" font="default" size="100%">Magnetism</style></keyword><keyword><style  face="normal" font="default" size="100%">Nuclease activity</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2018</style></year><pub-dates><date><style  face="normal" font="default" size="100%">MAR</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">473</style></volume><pages><style face="normal" font="default" size="100%">121-132</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Two new homometallic Cu4O4 cubane clusters 1 and 2 have been synthesized by self-assembly of copper (II) acetate and ligand, 2-[(2-Hydroxy-3-methoxy-benzylidene)-amino]-2-hydroxymethyl-propane-1, 3-diol (H4L) and characterized thoroughly by various spectroscopic techniques and single crystal X-ray diffraction analysis. Temperature- dependent magnetic susceptibility measurements have been performed to elucidate the antiferromagnetic and ferromagnetic nature in Cu4O4 clusters 1 and 2, respectively. In vitro DNA binding studies of cubane clusters were carried out by employing optical spectroscopic techniques. Gel electrophoretic mobility assay performed to examine the nuclease activity of the complexes 1 and 2 with pBR322 DNA, and results revealed oxidative DNA cleavage via reactive oxygen species (ROS) species viz., O-2(.-), O-1(2), etc. In vitro cell proliferation via MTT assay was studied to calculate the cytotoxicity of complexes 1 and 2. The IC50 evaluated were similar to 20 mu M in MCF-7 (Breast) and similar to 30-35 mu M in HepG2 (Liver) cancer cell lines. Additionally, in the presence of 1 and 2, ROS and TBARS (Thiobarbituric acid reactive substance) levels amplified significantly, coupled with GSH (glutathione) levels in cancer lines. Hence, the results exhibited the major role of ROS in apoptosis induced by 1 and 2 clusters and validate their prospective to be efficient anticancer drug entities. (C) 2018 Elsevier B.V. All rights reserved.</style></abstract><custom3><style face="normal" font="default" size="100%">Foreign</style></custom3><custom4><style face="normal" font="default" size="100%">2.002</style></custom4></record></records></xml>