<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Atul, Kumar</style></author><author><style face="normal" font="default" size="100%">Vivek, Lonkar</style></author><author><style face="normal" font="default" size="100%">Archana, Pundle</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Studies on optimization of cultural conditions and effect of media constituents on production of intracellular penicillin V acylase from Erwinia aroideae (DSMZ 30186)</style></title><secondary-title><style face="normal" font="default" size="100%">Research Journal of Biotechnology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">carbon sources</style></keyword><keyword><style  face="normal" font="default" size="100%">Erwinia aroideae</style></keyword><keyword><style  face="normal" font="default" size="100%">intracellular</style></keyword><keyword><style  face="normal" font="default" size="100%">nitrogen sources</style></keyword><keyword><style  face="normal" font="default" size="100%">Penicillin V acylase</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2008</style></year><pub-dates><date><style  face="normal" font="default" size="100%">MAY</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">2</style></number><publisher><style face="normal" font="default" size="100%">RESEARCH JOURNAL BIOTECHNOLOGY</style></publisher><pub-location><style face="normal" font="default" size="100%">SECTOR A-80, SCHEME NO 54, VIJAY NAGAR, A B ROAD, INDORE, 452 010 MP, INDIA</style></pub-location><volume><style face="normal" font="default" size="100%">3</style></volume><pages><style face="normal" font="default" size="100%">26-30</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Penicillin V acylase (PVA) is a pharmaceutically important enzyme. Erwinia aroideae (DSMZ 30186) produced high levels of intracellular penicillin V acylase. Amongst various carbon sources used (2.0 %); fructose, galactose, sucrose and mannitol increased enzyme production up to 579, 544, 534 and 504 IU/g dry weight (DW), respectively, compared to minimal media (263 IU/g DW); however maximum PVA productivity (2796 IU/ L) was achieved using fructose. Ammonium ions escalated the enzyme production to 1266.9 IU/g DW; however sodium glutamate was the best nitrogen source for overall productivity of enzyme (2045 IU/ L). Cornsteep liquor and skim milk were used as supplements; 1.0 % cornsteep liquor and 3.0 % skim milk were optimum for the production of PVA up to 823 and 93 IU/g DW, respectively. Optimum cultural conditions for the production of PVA from E. aroideae were standardized. Erwinia aroideae produced 1543 IU/g DW penicillin V acylase in Erlenmeyer flasks (250 ml) containing 50 ml of minimal medium with 0.3% ammonium sulphate, pH 7. 0 at 28 degrees C and 180 rpm when incubated for 56 hr.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><custom3><style face="normal" font="default" size="100%">Indian</style></custom3><custom4><style face="normal" font="default" size="100%">0.284</style></custom4></record></records></xml>